pH RESPONSIVE CASEIN MICROPARTICLES AS A CARRIER FOR METHOTREXATEAbstract
Casein microparticles cross linked with glutaraldehyde were prepared by steric stabilization process and the controlled release of drug from the particles were evaluated in-vitro. Casein is a natural biopolymer present abundantly in milk is utilized because of its biocompatibility through oral route. Methotrexate loaded Casein microparticles of size 0.5 to 5 µm were prepared. The release of methotrexate from the casein microparticles were evaluated in simulated gastric and intestinal fluids in-vitro. The coating of the particles with Eudragit L 100 made them as pH sensitive and therefore we found a significant release in the intestinal pH. The particles were resistant to proteolytic activity which can increase the durability of the particles in the intestine. This study demonstrates that the casein microparticles can be made pH sensitive and it can be successfully used as a targeted delivery system.
S. Vino*, K. R. Lokesh, S. Vijayaragavan, G. Jayaraman and A. R. Ghosh
School of Bio Sciences and Technology, VIT University, Vellore, Tamil Nadu, India
28 September, 2010
22 November, 2010
17 January, 2011
01 February, 2011