PHYTO-FABRICATION OF SILVER NANOPARTICLES BY DIFFERENT PHYSICO-CHEMICAL PARAMETRES USING PETREA VOLUBILIS L. LEAF BROTH AND EVALUATION OF THEIR ANTI-MICROBIAL ACTIVITYAbstract
The present study instantiates the phyto-fabrication of silver nanoparticles by utilizing Petrea volubilis L. leaf broth which were instantaneously synthesised, eco-friendly, and cost-effective. The influence of physico-chemical parameters viz. contact time, leaf broth quantity, pH, temperature, and silver nitrate concentration were investigated and optimised to engineer infinitesimal nanoparticles. The SPR bands obtained from UV-vis spectroscopy confirmed the synthesis of silver nanoparticles. The active phytochemicals in Petrea volubilis L. leaf broth namely carboxylic acids, phenolic compounds and aldehydes were found to be responsible for the bio reduction and capping of silver ions according to FT-IR spectroscopy. The capping molecules of NPs were negatively charged and were passably stable as revealed by zeta potential measurements and the XRD patterns illustrated their crystallinity. HRTEM microphotographs enumerate spherical shaped nanoparticles. The EDS analysis at 2θ confirms the synthesis of elemental silver. The nanoparticles were evaluated for their antimicrobial activity. The highest anti-bacterial activity was observed against S. aureus as compared to the bacteria S. typhi and A. flavus a fungus. The leaf extract did not show any anti-microbial activity. Our findings disclose the synthesis of various sizes of silver nanoparticles by varying the physico-chemical parameters. These diminutive nanoparticles are promising antimicrobial agents and evince a great potential in medical applications.
N. I. Hulkoti* and T. C. Taranath
P.G. Department of Studies in Botany, Environmental Biology Laboratory, Karnatak University, Dharwad, Karnataka, India.
06 March, 2017
29 April, 2017
27 May, 2017
01 October, 2017