PHYTOCHEMICAL, CYTOTOXICITY AND DOCKING STUDIES OF SAMANEA SAMANAbstract
Samanea saman is a tropical avenue tree which is widely cultivated in Northern America, and Asia belongs to the family Leguminosae. It possesses a wide range of biological perspectives. Folklore claims postulated that Samanea saman is having an antiulcer, antioxidant antibacterial and anti-mycobacterial properties. In the present investigation, the methanol extract of leaves of Samanea saman has been prepared based on the polarity and subjected to column chromatography. Based on this principle, the bioactive alcoholic extract (5%) was fractioned over a Silica gel (100-200 mesh) column by eluting with solvents of increasing polarity in the order of benzene: ethyl acetate and methanol. Similar TLC fractions were being combined and characterized by spectral analysis. Spectral data are revealed the presence of phytocompounds from the methanolic fractions of the Samanea saman. It was found that the compound named Pithecolobine showed remarkable activity against Mycobacterium tuberculosis by screening through Alamar blue assay (MIC at 3.12 µg/ml) and Luciferase assay (86% inhibition at 10 µg/ml) apart from other compounds obtained. The compound was also effective against lung cancer associated with tuberculosis. Further, the compound Pithecolobine was subjected to virtual screening. The docking studies of pithecolobine against the target proteins Enoyl-acyl carrier protein reductase (ENR) and Decaprenylphospho-beta-D-ribofuranose 2-oxidase (DprE1) reveals the compound pithecolobine is a potential drug candidate for tuberculosis.
G. S. Kumar * and S. D. S. Kumaran
Seven Hills College of Pharmacy, Venkatramapuram, Tirupati, Andhra Pradesh, India.
22 June 2018
31 August 2018
19 January 2019
01 March 2019