PHYTOCHEMICAL SCREENING AND HPTLC FINGERPRINTING PROFILE OF STEM BARK EXTRACTS OF MAYTENUS EMARGINATAAbstract
Objective: To establish the preliminary phytochemical screening and fingerprint profile of stem bark Extracts of Maytenus emarginata by using high-performance thin layer chromatography (HPTLC) technique. Methods: Preliminary phytochemical screening was done, physical constants were evaluated, and HPTLC studies were carried out. CAMAG make HPTLC system equipped with Linomat 5 applicator, TLC scanner 3, server vision CATS-server PH, version 2.5.18072.1 software were used. Results: Preliminary phytochemical screening of the extract showed the presence of flavonoids, glycosides, tannins, alkaloids, terpenoids, phenolic compounds, proteins, reducing sugars, fats, and oils. The fingerprint analysis of petroleum ether extract showed four peaks; chloroform extract showed four peaks and methanolic extract showed six peaks in 10 μl of the sample analyzed. Conclusions: It can be concluded that HPTLC fingerprint analysis of Petroleum ether, chloroform and methanolic extract of stem bark of Maytenus emarginata can be used as a diagnostic tool for the correct identification of the plant and it is useful as a phytochemical marker and also a good estimator of genetic variability in plant populations.
U. P. Joshi * and R. D. Wagh
Department of Pharmacognosy, DCS's A. R. A. College of Pharmacy, Nagaon, Dhule, Maharashtra, India.
03 November 2018
14 January 2019
18 January 2019
01 July 2019