PHYTOCHEMICAL SCREENING AND IN-VITRO ANTIOXIDANT ACTIVITY OF KNEMA ATTENUATA (HOOK. F. & THOMSON) WARB STEM BARK EXTRACTAbstract
The plant Knema attenuata or Myristica attenuata (Myristicaceae) is an endemic tree species found in the Western Ghats. Traditionally, the stem bark of K. attenuata is used in folk medicine without any scientific information available for the same. Phytochemical screening was carried out for ethanolic stem bark extract of K. attenuata to investigate the antioxidant potential of the extract. The antioxidant activity of the extract was estimated employing in-vitro models: DPPH radical scavenging assay, total antioxidant activity, nitric oxide scavenging assay and reducing power activity. The extract was found to be rich in phenolics, alkaloids, and flavonoids. Quantitative estimation of extract demonstrated higher levels of phenolics and flavonoids. The extract exhibited significant radical scavenging activity and a total antioxidant capacity compared with that of ascorbic acid (Standard) which confirms the use of the species in folk medicine.
H. S. Raja * and J. Suku
Department of Pharmacology, College of Pharmaceutical Sciences, Trivandrum, Kerala, India.
27 October 2018
14 January 2019
22 July 2019
01 September 2019