PRECLUSION OF HYPOXIA: IDENTIFICATION OF POTENTIAL INHIBITOR AGAINST HIF-1 ALPHA PROTEIN THROUGH MOLECULAR DOCKINGAbstract
Low level of oxygen in tissues leads to hypoxia, and hypoxia-inducible factors (HIF) regulate the hypoxic and normoxic conditions in various tissues. During normoxia, HIF-1α binds to von Hippel-Lindau E3 ubiquitin ligase complex that targets HIF-1α to the ubiquitin-proteasome pathway for proteolytic destruction. But in hypoxia, HIF-1α move to the nucleus where it binds to CBP/p300 at CH1 domain. By considering this fact, the present study was conducted to search a suitable inhibitor that can bind to HIF-1α. The unliganded HIF-1α was docked and the best five docking solutions complex were selected and analyzed by Ligplot. The analysis showed that catechin, epicatechin, myricetin, dicarnoxide D, and pycnidione had the maximum potential to inhibit HIF-1α protein and may prove to be potential inhibitor for counterfeiting hypoxic conditions.