PREPARATION AND PHYSICAL CHARACTERIZATION OF CISPLATIN CHITOSAN NANOPARTICLES BY ZETA NANOSIZER “PRIME STEP FOR FORMULATION AND DEVELOPMENT”Abstract
The ultimate aim of this research work is to develop a cisplatin loaded chitosan nanoparticle system (CCNP) to facilitate the release of cisplatin in a more sustained manner to improve therapeutic efficacy via the reduction of its toxicity. As a step-in formulation studies the work has been limited to studying the various physical characters of CCNP using a zeta nanosizer. The chitosan polymer was selected in this study because of the high poly-cationic, biodegradable and biocompatible characters. The drug of choice is cisplatin, a well-known anticancer drug, used in the treatment of various cancer types. CCNP was prepared using the ionic gelation technique and characterized using the Malvern zetasizer nano zs instrument, UK to find out the polydispersity index (pdi), zeta potential and particle size determination. The ideal nanoparticles were exhibiting + 45.4 mV, 595.3 z.dnm and 0.6 pdi. The study concluded that CCNP can be formulated at an optimum concentration of 40 µg/ml of cisplatin entrapped in 2 mg/ml of aqueous solution of chitosan in the presence of formaldehyde (0.1% v/v) as cross linker and identification of physical characterization of nanoparticles was established as a preliminary step in formulation studies to target cancer cells.
H.A. Abouelhag, S.M. Sivakumar *, U. S. Bagul, E. M. Eltyep and M.M. Safhi
Division of Immunology and Pharmaceutical Biotechnology Department of Pharmaceutics College of Pharmacy, Jazan University, P.O. Box 114, Jazan, KSA
03 March, 2017
25 April, 2017
27 May, 2017
01 October, 2017