PRODUCTION AND CHARACTERIZATION OF CURDLAN FROM AGROBACTERIUM SP.Abstract
Biopolymers are gaining more importance in our day-to-day life, due to the hazardous effect of synthetic plastics in our environment. Curdlan is one of the biopolymers, which has a broad field of applications in the food, pharmaceutical and medical. It is a polymer of glucose, produced by the family members of Rhizobiaceae. Curdlan [(1→3)-β-D-glucan] production takes place under the nitrogen starvation period during the cell growth. Fifty Agrobacterium sp. were isolated from root nodules collected from different localities of Coimbatore and Palakkad and screened by Aniline Blue method for the production of curdlan. The species was confirmed as Agro-bacterium fabrum by 16S rRNA sequencing. The accession number given by GenBank is MF521602. Curdlan was produced using different carbon and nitrogen sources. The extraction was done by Sodium hydroxide: Acetic acid precipitation. 0.12 gm of crude curdlan was obtained from 1.2 gm/100 ml of biomass. The product was stored for further research.
V. Muthu Laxmi *, D. Latha and A. S. Jayasree
Division of Microbiology, School of Biological Sciences, CMS College of Science and Commerce, Chinnavedampatti, Coimbatore, Tamil Nadu, India.
03 March, 2018
17 May, 2018
18 June, 2018
01 November, 2018