QMS VERSUS HPLC-UV FOR LAMOTRIGINE PLASMA MONITORINGAbstract
Lamotrigine (LTG), a synthetic anticonvulsant, may lead to therapy failure or toxicity risks due to the wide inter and intra individual pharmacokinetic variations. Those wide variations in LTG require its therapeutic drug monitoring (TDM). Our study aims to compare the QMS method with the HPLC-UV which is considered as a reference method for TDM of LTG. The study was conducted in the Clinical Pharmacology Department of the National Centre of Pharmacovigilance during a period of nine months. It included 37 samples from 37 patients treated with LTG. The samples were monitored by HPLC-UV and by PETIA. Regression analysis performed with data yielded to the equation LTGPETIA = 0.7701 LTGHPLC + 1.475 with a Pearson correlation r = 0.804 (P<0.0001). Bias mean between the two methods was 13.45%. In conclusion, the turbidometric technique (QMS) is comparable to the HPLC-UV method in the interval of therapeutic concentration. Under low concentrations, the QMS technique is less precise.
E. Gaïes, M. Bouhlel *, R. Charfi, N. Jebabli, N. Baazaoui, I. Salouage, H. El-Jebari, A. Klouz and S. Trabelsi
Department of Clinical Pharmacology, National Centre of Pharmacovigilance, Tunis, Tunisia.
14 September, 2016
18 October, 2018
11 November, 2018
01 December, 2018