QUANTIFICATION OF β-SITOSTEROL FROM FIELD GROWN PLANTS AND CALLUS OF CRATAEVA TAPIA L.Abstract
Crataeva tapia L. (Family- Capparaceae) is an important medicinal tree, commonly called as ‘Varuna’. The plant has been reported to possess several medicinal properties. Propagation of Crataeva tapia L. is mostly by seeds, but germination percentage is very low mainly due to presence of hard seed coat. Conservation and propagation of species using biotechnological tools like plant tissue culture are relevant when natural propagation is hampered. Plant cell culture systems have potential for commercial exploitation of secondary metabolites. These secondary metabolites can then be analyzed using chromatographic techniques. In the present study, a simple, precise, rapid and selective High Performance Thin Layer Chromatography (HPTLC) method was developed and validated for detection and quantification of phytochemical marker β-sitosterol in leaf, bark, leaf callus and stem callus of Crataeva tapia L. as per ICH guidelines.
Priyanka Sharma*, Avinash Patil and Darshana Patil
Department of Botany 1 Birla College, Kalyan, Mumbai, Maharashtra, India
19 October, 2015
13 December, 2015
21 January, 2016
01 April, 2016