SAFETY EVALUATION OF GALANGAL (ALPINIA GALANGA) EXTRACT FOR THERAPEUTIC USE AS AN ANTIMICROBIAL AGENTAbstract
Hexane extract of Galangal (Alpinia galanga) rhizome was evaluated for acute dermal, oral and intraperitoneal toxicities using OECD guidelines. The undiluted crude galangal extract showed negligible irritation on non-abraded skin of New Zealand white rabbits with 0.25 primary irritation index whereas the abraded skin of the rabbits showed irritation for all tested dilutions of galangal extracts; 0.75 g/ml, 0.5 g/ml, 0.25 g/ml and 0.125 g/ml. The single oral dose of the Galangal extract at 2000 mg/kg did not produce mortality or significant changes in the general behavior, body weights, feed intake and biochemical analysis (ALT, AST, BUN and creatinine levels) of Wistar rats compared to the control. However, 2000 mg/kg and 50 mg/kg body weight of galangal extract were highly toxic to Wistar rats when administered intraperitoneally. Galangal extract with concen-trations <20 mg/ml were non-irritant on Hen’s egg chorioallantoic membrane which is an alternative to the Draize eye irritation test.
P. U. H. S. Karunarathne, M. G. Thammitiyagodage * and N. S. Weerakkody
Department of Laboratory Animal Science, Medical Research Institute, Colombo 08, 00800, Western Province, Sri Lanka.
24 February, 2018
23 May, 2018
31 May, 2018
01 November, 2018