SIMULTANEOUS DETERMINATION OF PHENOLIC COMPOUNDS IN CATHARANTHUS ROSEUS LEAVES BY HPLC METHODAbstract
In Catharanthus roseus, Rutin, Quercetin and Kaempferol are the most important phenolic compounds with antioxidant activity. For simultaneous determination of Rutin, Quercetin and Kaempferol in Catharanthus roseus a simple and rapid HPLC method was developed. Phosphate buffer (pH=5.8) and solvent Acetonitrile are used in ratio of 55: 45 as mobile phase. Rutin, Quercetin and Kaempferol were determined by HPLC by using Athena C18 column and UV absorbance were measured at 254 nm. Retention time (Rt) of standards, Rutin, Quercetin and Kaempferol was found to be 2.357, 6.093 and 9.373 respectively, while in Catharanthus roseus the Retention times of Rutin, Quercetin and Kaempferol are 2.403, 6.143 and 8.903 are found to be matching with standards Retention time values. Thus for quantitative estimation of phenolic compounds, this HPLC method was found to be simple and convenient.
A. Srinivasa Rao* and Mohammed Fazil Ahmed
Bhaskar Pharmacy College, Yeknapally, Moinabad (Mandal), R.R (Dist), Hyderabad 500075, Andhra Pradesh, India
24 October, 2013
04 December, 2013
16 February, 2014