SIMULTANEOUS ESTIMATION OF PARACETAMOL AND TAPENTADOL IN COMBINED DOSAGE FORM BY DERIVATIVE METHODAbstract
Two simple, rapid, precise and accurate spectrophotometric methods have been developed for simultaneous analysis of Paracetamol (PCM) and Tapentadol (TAP) in their combined dosage form. Method A, First order derivative method involves measurement of absorbance at 257.060 nm (for TAP) and 289.406 nm (for PCM) in first order derivative spectra. Method B, Second order derivative method involves measurement of absorbance at 238.391 nm (for TAP) and 279.381 nm (for PCM) in Second order derivative spectra. Developed methods were validated according to ICH guidelines. The calibration graph follows Beer’s law in the range of 1.0 to 5.0 μg/mL for Tapentadol and 6.5 to 32.5 μg/mL for Paracetamol with R square value greater than 0.997 (for Method A) and 0.998 (for Method B). Accuracy of all methods was determined by recovery studies and showed % recovery between 98 to 102%. Intraday and inter day precision was checked for all methods and mean %RSD was found to be less than 2 for all the methods. The methods were successfully applied for estimation of Paracetamol and Tapentadol in marketed formulation.
Vishal Khokhar* and R. M. Shah
Department of Quality Assurance, N. R. Vekaria Institute of Pharmacy, C. L. College Campus, Bilkha Road, Junagadh-362001, Gujarat, India
07 January, 2013
04 March, 2013
27 April, 2013
01 May, 2013