SPECTROFLUORIMETRIC DETERMINATION OF TOLTERODINE TARTARATE IN PURE FORM AND PHARMACEUTICAL PREPARATIONAbstract
A Simple, rapid, sensitive, accurate and precise spectrofluorimetric method was developed for the determination of tolterodine tartrate (TOL) in bulk powder and in pharmaceutical preparation. This method is based on measuring the native fluorescence of TOL in methanol at 313 nm after excitation at 285 nm using 1 ml of phosphate buffer pH 6. All variables that affect fluorescence intensity such as diluting solvents, pH, buffer and buffer volume were studied and optimized .The fluorescence-concentration plot was rectilinear over the range of 0.5-8 µg/ml with a lower detection limit (LOD) of 0.090 µg/ml and lower quantification limit (LOQ) of 0.273 µg/ml. The method was validated and successfully applied to the determination of TOL tablets with an average percent recovery ± RSD% of 100.57 ± 0.76. The obtained results were statistically compared with those of the reported method by applying t-test and F-test at 95% confidence level and no significant difference was observed regarding accuracy and precision.
Mohammed W. Nassar, Khalid A. Attia, Hamed M. Abou-Seada and Ahmed El-Olemy Allam*
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt
19 May, 2013
01 August, 2013
26 September, 2013
01 October, 2013