STABILITY INDICATING RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF SALBUTAMOL AND BECLOMETHASONE IN BULK AND TABLET DOSAGE FORMHTML Full Text
STABILITY INDICATING RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF SALBUTAMOL AND BECLOMETHASONE IN BULK AND TABLET DOSAGE FORM
Nandini Abbai * and S. Angala Parameswari
Department of Pharmaceutical Analysis, Krishna Teja Pharmacy College, Tirupathi - 517520, Andhra Pradesh, India.
ABSTRACT: A new, simple, sensitive, precise, accurate and stability indicating RP-HPLC method has been developed for the simultaneous estimation of salbutamol and beclomethasone in combined tablet dosage form. The analysis was carried out at 230 nm and this chromatographic separation was achieved with Kromasil 250 C18 [250 x 4.6 mm, 5µ] column under 25 oC temperature and using mobile phase water and acetonitrile in a ratio of 55:45 v/v adjusted pH 2 with ortho phosphoric acid. The retention times of salbutamol and beclomethasone were found to be 2.030 ± 0.01 min, 3.271 ± 0.1min respectively. The proposed method was validated according to ICH guidelines. The linearity study of salbutamol and beclomethasone was found in concentration range of 25 - 150 µg/ml and 50 - 300 µg/ml and correlation coefficient (r2) was found to be 0.999 and 0.999. The percentage recovery was obtained as 98.61 % and 100.75 % w/v for beclomethasone and salbutamol. The LOD, LOQ values were obtained from regression equations of beclomethasone and salbutamol were 1.13 µg/ml, 2.06 µg/ml and 3.42 µg/ml, 6.23 µg/ml respectively. The studies were carried out by conducting deliberate degradation of the sample with exposure to stress conditions like acidic (1M HCl), alkaline (1M NaOH), thermal, oxidizing agents (H2O2) and water. This method was validated and met the regulatory requirements for specificity, Linearity, LOD, LOQ, Precision, accuracy and stability for the determination of salbutamol and beclomethasone in bulk and tablet dosage form by RP-HPLC.
Beclomethasone, Salbutamol, Simultaneous estimation RP-HPLC
INTRODUCTION: Salbutamol is chemically 4-[2-(tert-butylamino)-1-hydroxyethyl] - 2- (hydroxyl methyl) phenol. It is a short-acting, selective beta-2-adrenergic receptor agonist used in the treatment of asthma and COPD. Salbutamol is generally used for acute episodes of bronchospasm caused by bronchial asthma, chronic bronchitis and other chronic broncho pulmonary disorders such as chronic obstructive pulmonary disorder (COPD).
It is also used prophylactically for exercise induced asthma. Beclomethasone is chemically 2-[(1R, 2S,10S,11S,13S,14R,15S,17S)-1-chloro-17 - hydro xy-2,13,15-trimethyl-5-oxo-14-(propanoyloxy) tetra cyclo heptadeca-3,6-dien-14-yl]-2-oxoethyl propa- noate. Beclomethasone dipropionate is a prodrug of the free form, it is anti-inflammatory, synthetic corticosteroid and it is used topically as an anti-inflammatory agent and in aerosol form for the treatment of asthma and allergic rhinitis. These researches focus the simultaneous estimation of salbutamol and beclomethasone in API or combined tablet form by RP - HPLC.
The extensive literature survey revealed that there were RP-HPLC 1 - 3 and UV spectrophotometric 4, stability indicating RP - HPLC 5 methods were available for the determination of salbutamol and beclomethasone individually or in combination with other drugs 6 - 15. But no method was reported for simultaneous estimation of salbutamol and beclomethasone in combined dosage form using RP-HPLC method. The study was thus performed with an aim to develop a simple, economic, sensitive, rapid, accurate and precise method for the determination of salbutamol and beclomethasone in combined tablet dosage form.
FIG. 1: STRUCTURE OF SALBUTAMOL
FIG. 2: STRUCTURE OF BECLOMETHASONE
MATERIALS AND METHODS:
Chemicals and Reagents: Salbutamol and Beclomethasone were purchased from Spectrum Labs, Hyderabad. Orthophosphoric acid (OPA) purchased from SD Fine Chemicals (Hyderabad, India), methanol, acetonitrile and HPLC grade water were obtained from Rankem. Salbutamol and beclomethasone containing aerotide tablets manu-factured by Sun Pharmaceutical Ltd, Hyderabad, India.
Instruments: Waters (2695) HPLC using the software Empower 2. All the glass wares used were ‘A’ grade.
Standard stock solution: 10 mg of salbutamol and 20 mg of beclomethasone standards were accurately weighed and transferred into a 10 ml clean, dry volumetric flask, 5 ml of diluent was added, sonicated for 5 minutes and made up to the final volume with diluent.1ml from the above stock solution was taken into a 10 ml volumetric flask and made up to 10ml with diluent.
Preparation of Sample Solution: 20 tablets were weighed and crushed. A powder equivalent to 10 mg of salbutamol and 20 mg of beclomethasone was taken and then transferred into a 10 ml clean, dry volumetric flask, 5 ml of diluents was added, sonicated for 5 minutes and made up to the final volume with diluent. 1 ml of the above stock solution was taken into a 25 ml volumetric flask and made up to 25 ml with diluent.
Chromatographic Conditions: Kromasil 250C18 [250 x 4.6 mm, 5µ] column was used for the chromatographic separation at a detection wave length of 230 nm, under 25 oC temperature. Mobile phase of buffer and acetonitrile pH 2 (adjusted with OPA) in a ratio of 55:45 v/v which was degassed under ultra-sonication was selected for elution and same mixture was used in the preparation of standard and sample solutions. Flow rate was optimized to 0.9 ml/min and the injection volume 10µlwas fixed upon the satisfactory results of various system suitability parameters such as retention time, column efficiency, tailing factor, asymmetry of the peaks. The results were shown in Fig. 3.
Method Validation: The RP-HPLC method was validated according to ICH guidelines for validation of analytical procedures for different validation parameters. The method was validated for its specificity, linearity, accuracy, precision, robustness, ruggedness, LOD and LOQ.
System Suitability: System suitability test was carried out to verify that the analytical system was working properly and can give accurate and precise results. The overall system suitability was evaluated for the system suitability of the proposed method. Data from six injections (10 μg/mL) were utilized for calculating parameters like theoretical plates, resolution, tailing factor and %RSD. The results were shown in Table 2.
Specificity: The specificity studies were carried out by varying specific conditions, i.e., placebo study. A study conducted to demonstrate that diluent and Placebo were not interfering with the analyte peak in the proposed method. Solutions of sample, placebo and blank were prepared individually and chromatograms were obtained. The results were shown in Fig. 3, 4 and 5.
Accuracy: Accuracy was carried out by % recovery studies of salbutamol and beclomethasone sat three different concentration levels (50 %, 100 %, and 150 %). Percentage recovery was calculated from the amount added and the amount recovered. The percentage recovery was within the acceptance criteria, this indicates the accuracy of the method. (Acceptance criteria: % recovery between 98 - 102%). The results were shown in Table 3.
Precision: Precision of an analytical procedure as the closeness of agreement between a series of measurements obtained from multiple sampling of the same homogeneous sample under the prescribed conditions. The results were shown in Table 4.
Linearity: Linearity of an analytical procedure is its ability (within a given range) to obtain test results that are directly proportional to the concentration (amount) of analyte in the samples. The linearity of the method was determined by preparing serial dilutions of minimum 5 concentrations of working stock solutions in the range of 50 - 300 μg/ml for beclomethasone and 25 - 150 μg/ml for salbutamol. The area of each injection was obtained and the peak area was plotted against actual concentration. The regression coefficient r2, y-intercept and slope of the regression were calculated.
Limit of Detection and Limit of Quantitation: The LOD is defined as the lowest concentration of an analyte in a sample that can be detected but not quantified. The LOQ is defined as the lowest concentration of an analyte in a sample that can be determined with acceptable precision and accuracy. The LOD and LOQ can be calculated based on the standard deviation of the response and the slope of calibration curve. LOD=3.3σ/S LOQ=10σ/S where‘σ’ is the standard deviation of the intercept of the regression lines and ‘s’ cis the slope of the calibration curve.
Robustness: The robustness of an analytical variation in method parameters such as flow rate, column temperature were varied within a realistic range and the quantitative influence of the variables were determined. The results were shown in Table 5 and Table 6.
Stability Studies: Stability testing was established for estimating the allowed time span between sample collection and sample analysis. It is also important to evaluate an analytical method’s ability to measure drug products in the presence of its degradation products. Forced degradation studies typically involve the exposure of samples of the drugs to the relevant stress conditions of acid, base, thermal and UV. The results were shown in Table 7.
Acid Degradation: To 1 ml stock solution of salbutamol and beclomethasone, 1ml of 2N hydro-chloric acid was added and refluxed for 30 mins at 60 ͦ C. The resultant solution was diluted to obtain 100 µg/ml and 200 µg/ml solutions and 10µl solutions were injected into the system and the chromatogram were recorded to assess the stability of drug substances. The results were shown in Fig. 9.
Oxidation Degradation: To 1 ml of stock solution of salbutamol and beclomethasone, 1 ml of 20 % hydrogen peroxide (H2O2) was added separately. The solutions were kept for 30 min at 60 ͦ C. For HPLC study, the resultant solution was diluted to obtain 100µg/ml and 200 µg/ml solution and 10µl were injected into the system and the chromato-grams were recorded to assess the stability of drug substances. The results were shown in Fig. 10.
Alkali Degradation: To 1 ml of stock solution of salbutamol and beclomethasone, 1 ml of 2N sodium hydroxide was added separately. The solutions were kept for 30 min 60 ͦ C. For HPLC study, the resultant solution was diluted to obtain 100µg/ml and 200µg/ml solution and 10µl were injected into the system and the chromatograms were recorded to assess the stability of drug substances. The results were shown in Fig.11.
Thermal Degradation: The standard drug solution was placed in oven at 105 °C for 6hr to study dry heat degradation. For HPLC study, the resultant solution was diluted to obtain 100µg/ml & 200µg/ml solution and10µl were injected into the system and the chromatograms were recorded to assess the stability of drug substances. The results were shown in Fig. 12.
Photo Degradation: The photochemical stability of the drug was also studied by exposing the 1000 µg/ml and 2000 µg/ml solution to UV light by keeping the beaker in UV Chamber for 7 days or 200 Watt hours/m2 in photo stability chamber. For HPLC study, the resultant solution was diluted to obtain 100µg/ml and 200 µg/ml solutions and 10µl were injected into the system and the chromatograms were recorded to assess the stability of drug substances. The results were shown in Fig. 13.
RESULTS AND DISCUSSION: Optimization of the method was carried out by performing various trials by change in mobile phase composition, column and flow rate etc. The chromatographic conditions were achieved by water and acetonitrile in a ratio of 55:45 v/v adjusted pH 2 with ortho phosphoric acid was selected as mobile phase and Kromasil 250C18 [250 x 4.6 mm, 5µ] column as stationary phase because of better resolution, number of Theoretical plates and symmetric peaks. Salbutamol and beclomethasone were found to show appreciable absorbance at 230 nm determined spectro-photometrically and hence it was selected as the detection wavelength. Fig. 3 represents chromate-gram of mixture of standard solutions.
TABLE 1: OPTIMIZED CHROMATOGRAPHIC PARAMETERS
|Optimized chromatographic conditions|
|Mode of separation||Isocratic|
|Mobile phase||Water : acetonitrile (55:45)|
(250 x 4.6 mm, 5m)
|Column temperature||25 ˚C|
|Detection wavelength||230 nm|
|Run time||6 min|
|Flow rate||0.9 ml/min|
System Suitability: An RP -HPLC method was developed by monitoring the system suitability parameters, i.e. Tailing factor (T), the number of theoretical plates (N), the runtime and the cost effectiveness. System suitability method acceptance criteria set in each validation run were: tailing factor ≤ 2.0 and theoretical plates > 2000. In all cases, the relative standard deviation (R.S.D) for the analytic peak area for two consecutive injections was < 2.0%. A chromatogram obtained from reference substance solution was presented. System suitability parameters were shown in Table 2. All the system suitability parameters are found to be satisfactory. The peak is reasonably symmetrical. High numbers of theoretical plates indicate the efficient performance of the column with reasonable retention times.
Specificity: The blank chromatogram showed no interference peaks at the retention time of beclomethasone and salbutamol. This indicates that diluent solution used in sample preparation do not interfere in the estimation of beclomethasone and salbutamol. Similarly the placebo sample chromatogram showed no interference peaks at the retention time of beclomethasone and salbutamol respectively, which indicates the specificity of the proposed method. The results were given in Fig. 4, 5 and 6.
TABLE 2: RESULT OF SYSTEM SUITABILITY PARAMETERS
|Linearity range (µg/ml)||50-300 µg/ml||25 - 150 µg/ml|
|Retention time (RT)||3.267min||2.032 min|
|Number of theoretical
|Correlation coefficient (r2)||0.999||0.999|
|Tailing factor (T)||1.2||1.2|
|Limit of detection (µg/ml)||1.13||2.06|
FIG. 4: CHROMATOGRAM OF BLANK
FIG. 5: CHROMATOGRAM OF PLACEBO
FIG. 6: CHROMATOGRAM OF STANDARD
The blank and placebo peaks are not interfering with the analyte so the method was specific.
Linearity: The concentration range of 50-300μg/ml for beclomethasone and 25-150 μg/ml of Salbutamol were found to be linear with correlation coefficients 0.999 and 0.999 for beclomethasone and Salbutamol respectively. The results were given in Fig.7 and 8.
Accuracy: The accuracy of an analytical method is the closeness of that results obtained by that method to the true value. Accuracy may often be expressed as percent recovery by the assay of known added amount of analyte. % Recovery
should be in the range of 98.0% to 102 %. The observed data was within the required range, which indicates good recovery values and hence the method was accurate. The results were shown in Table 3.
Precision: The percentage relative standard deviation was calculated for the peak areas of the drug and it was found to be 0.3 % and 0.7 % for salbutamol and beclomethasone respectively. The % RSD for the area of six standard injections were should not be more than 2 % and the method was found to be precised. The results were shown in Table 4.
FIG. 7: CALIBRATION PLOT OF BECLOMETHASONE
FIG. 8: CALIBRATION PLOT OF SALBUTAMOL
TABLE 3: DATA FOR ACCURACY
|Sample||The amount added (µg/ml)||Amount recovered (µg/ml)||Recovery (%)||% RSD|
TABLE 4: PRECISION RESULTS
Linearity: Linearity of detector response shows the linear relationship between the concentration and the detector response. The coefficient of salbutamol and beclomethasone correlation of was found to be 0.999.The linearity was found in the concentration range of 50 - 300 µg/ml of beclomethasone, 25 - 150 µg/ml of salbutamol.
Regression equation of the beclomethasone and salbutamol are found to be, y = 14964x + 61485, and y = 5778.7x + 3827.3 and the regression co-efficient was 0.999 respectively. The correlation coefficient value was < 1 shows that the method was linear.
Limit of Detection: Limit of detection of target assay concentration of salbutamol and beclometha-sone by using the formula method was found to be 2.06µg/ml and 1.13µg/ml respectively.
Limit of Quantification: Limit of quantification of the target assay concentration of salbutamol and beclomethasone by using the formula method was found to be 6.23µg/ml and 3.42 µg/ml respectively.
Robustness: Robustness of the method tested by keeping the ratio of mobile phase as constant and the chromatograms of drug solution were recorded with different flow rates such as 1.0 ml/min, 0.8 ml/min and 0.9 ml/ min and different column temperatures such as 20 °C, 25 °C, 30 °C. The peak was observed as sharp with good resolution and passes all system suitability parameters indicating the method was robust. The results were reported in Table 5 and 6.
TABLE 5: RESULT OF ROBUSTNESS STUDIES (FOR VARIENT FLOW RATE)
|Analytes||Flow rate(ml)||Plate count||Tailing|
TABLE 6: RESULT OF ROBUSTNESS STUDIES (FOR VARIENT COLUMN TEMPERATURE)
|Analytes||Column temperature (oC)||Plate count||Tailing|
Degrdation Studies: The degradation was determined by analyzing both drug solutions in presence of acid, base, hydrogen peroxide, thermal and UV. The results of peak area of salbutamol and beclomethasone was changed hence the drug was degredated but the % degradation was less than 10% w/v. The results were within the limit as per ICH guidelines shown in Table 7.
TABLE 7: FORCED DEGRADATION STUDY RESULTS FOR SALBUTAMOL AND BECLOMETHASONE
|Area||Recovery in %||% Degraded||Area||Recovery in %||Degraded Drug %|
FIG. 10: TYPICAL CHROMATOGRAM OF BASE DEGRADATION OF SALBUTAMOL AND BECLOMETHASONE
FIG. 11: TYPICAL CHROMATOGRAM OF PEROXIDE DEGRADATION OF SALBUTAMOL AND BECLOMETHASONE
FIG. 12: TYPICAL CHROMATOGRAM OFTHERMAL DEGRADATIONOF SALBUTAMOL AND BECLOMETHASONE
FIG. 13: TYPICAL CHROMATOGRAM OF UV DEGRADATION OF SALBUTAMOL AND BECLOMETHASONE
FIG. 14: TYPICAL CHROMATOGRAM OF SALBUTAMOL AND BECLOMETHASONE IN WATER
CONCLUSION: The study was undertaken in order to develop and validate the stability indicating RP-HPLC method for estimation of salbutamol and beclomethasone in pharmaceutical formulations.The method was developed and validated by means of accuracy, precision, linearity, LOD and LOQ and robustness as per ICH guidelines. The results of the study indicate that the proposed RP-HPLC method can be used in quality control departments with respect to routine analysis for the assay of the tablets containing salbutamol and beclomethasone.
ACKNOWLEDGEMENT: Author expresses sincere thanks to the Krishna Teja Pharmacy College for providing facility to carry out this work.
CONFLICTS OF INTEREST: Nil
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How to cite this article:
Abbai N and Parameswari SA: Stability indicating RP-HPLC method development and validation for simultaneous estimation of salbutamol and beclomethasone in bulk and tablet dosage form. Int J Pharm Sci Res 2018; 9(5): 1980-88.doi: 10.13040/IJPSR.0975-8232.9(5).1980-88.
All © 2013 are reserved by International Journal of Pharmaceutical Sciences and Research. This Journal licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.
N. Abbai * and S. A. Parameswari
Department of Pharmaceutical Analysis, Krishna Teja Pharmacy College, Tirupathi, Andhra Pradesh, India.
31 July, 2017
30 October, 2017
27 January, 2018
01 May, 2018