STANDARDIZATION WITH HPTLC ANALYSIS OF POLYHERBAL POWDER FORMULATION: SAFOOF-E-MUSAKKINAbstract
Background & Objectives: Standardization of polyherbal Unani formulations is essential to assess the quality of drugs, based on their active principles, physical and chemical standards. This work reports on standardization of a polyherbal Unani powder formulation Safoofe- Musakkin (SM) consist of Rauwolfia serpentina Benth. Ex kurz root, Coriandrum sativum Linn. seed and Piper nigrum Linn. fruit. It is used in neurological disorders. Methods and Material: Plant parts were first cleaned, dried in the shade and powdered by passing through sieve # no. 80 and prepared as per the method described in UPI. SM formulation was evaluated using physicochemical tests: powder characterization, extractive value, alcohol, and water-soluble matter, Ash value, LOD at 105 ºC, pH and HPTLC fingerprinting. Statistical Analysis Used: Mean ± SEM. Result: Organoleptic characters of SM revealed brown color, characteristic odor, bitter taste, and moderately fine texture. Physicochemical parameters resulted in water-soluble extractive (9.58 ± 0.11), alcohol-soluble extractive (6.29 ± 0.07), total ash (5.87 ± 0.09), acid insoluble ash (3.09 ± 0.08), water-soluble ash (1.5 ± 0.05), LOD at 105 ºC (7.75 ± 0.058), pH of 1% and 10% solution were 6.4 ± 0.1 and 5.6 ± 0.25 respectively. The phytochemical analysis shows the presence of alkaloids, glycosides, tannins, flavonoids, steroids, terpenoids, carbohydrates, sugars, volatile oil, saponins, anthraquinones, proteins, and phenols. HPTLC fingerprinting data in two mobile phases n-Butanol: Acetic acid: water (5:1:4) and Toluene: Ethyl acetate: Formic acid (5:4:1) (v/v) was set in. Conclusion: standardization data of SM was obtained as a standard and for future reference.
G. J. Rather, Hamidudin *, M. Ikram, M. Zaigham, G. Sofi and T. Nayak
Department of Ilmul Saidla (Unani Pharmacy), National Institute of Unani Medicine (NIUM), Bangalore, Karnataka, India.
07 September 2018
22 November 2018
24 November 2018
01 May 2019