SYNTHESIS OF SILVER NANOPARTICLES USING SWERTIPUNICOSIDE AND ITS ACTIVITY ON HUMAN BREAST CANCER CELL LINE (MCF-7)Abstract
In the current study, synthesis of silver nanoparticles (AgNPs) using swertipunicoside was tested against human breast cancer cell line (MCF-7). The swertipunicoside was tested against the normal cell line such as vero cells to find out the toxicity of the compound. The synthesized AgNPs were characterized using UV-visible spectroscopy, X-ray powder diffraction (XRD), fourier-transform infrared spectroscopy (FTIR) and field-emission Scanning Electron Microscope (FESESM) with energy dispersive spectroscopy (EDAX). The synthesized silver nanoparticles were checked with the colour variation and it was confirmed by UV-vis spectral analysis. The morphology of the synthesized nanoparticles were analysed using FESEM along with EDAX. The XRD was done to find out the crystalline structure of the compound. FTIR measurements are carried out to identify the possible biomolecules responsible for capping and efficient stabilization of the silver NPs synthesized using swertipunicoside. The synthesized AgNPs and swertipunicoside was tested against the MCF-7 cell line to find out the cell viability. Our results showed that the compound and synthesized silver nanoparticles inhibited the proliferation of human breast cancer cell line with an IC50 value of 60 mg/ml and IC50 of 40 µg/ml at 24 h incubation. From this study, concluded that swertipunicoside and synthesized AgNPs have potential anticancer activity.
S. Sivaranjani and C. Arulvasu *
Department of Zoology, University of Madras, Guindy Campus, Chennai, Tamil Nadu, India.
26 February, 2018
04 May, 2018
13 May, 2018
01 November, 2018