THE APPLICATION OF ULTRASOUND IRRADIATION AS AN ALTERNATIVE METHOD FOR THE PREPARATION OF 99mTc-OCTREOTIDE RADIO COMPLEXAbstract
This approach was launched to evaluate the application of ultrasound waves for the preparation of 99mTc-Octreotide radio conjugate sample. The 740MBq (20 mCi) of 99mTc-Octreotide samples were prepared by the boiling water bath (standard) or ultrasound irradiation methods. The stability of samples in normal saline and human serum albumin was investigated. The partition coefficient and protein bonding of samples were determined. The radioisotope analysis was undertaken to assess the biodistribution of radiotracer samples in rats. The data obtained from this approach revealed that the 99mTc-Octreotide samples were successfully prepared by the newly developed technique. Statistically significant differences have not been observed in the stability, partition coefficient and protein bonding of radiotracer samples which were prepared by either of the two above-mentioned. The radioisotope analysis showed that the applied method for radiolabeling did not lead to a significant difference in the biodistribution pattern of radiotracer samples in rats. Green chemistry can be recommended for the radiolabeling of Octreotide with 99mTc as an alternative and reliable modality for the boiling water bath method.
A. Doroudi *, M. Hemmati, S. M. Saadati, A. Kiasat, F. Ahmadi, B. Etesami, M. J. Khodayar and M. Erfani
School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
10 December 2018
18 March 2019
28 March 2019
01 September 2019