WOUND HEALING AND ANTIOXIDANT POTENTIAL OF CHLOROFORM EXTRACT OF SARACA ASOCA (ROXB.)Abstract
The chloroform extracts of S. asoca bark were studied for the wound healing potential. The wound healing activity of S. asoca bark by excision, incision and dead space wound models in albino Wistar rats and antioxidant profile of this bark extract were also studied as there is an increase in the levels of free radicals during the damage of tissue. The parameters like wound contraction, period of epithelialisation, tensile strength and hydroxyproline content were evaluated in three wound models, namely; excision, incision and dead space wound models. Besides, their influences on oxidative stress in three wound models were also estimated. The results obtained were compared with standard drug nitrofurazone and control in terms of wound contraction, period of epithelialisation, tensile strength and hydroxyproline content. The chloroform extract of S. asoca bark showed significant results in all models when compared to standard and control. A subservient study made on the levels of superoxide-dismutase, catalase, glutathione, vitamin C and lipid peroxidation are recorded and a significant increase in the levels of these antioxidant enzymes and decrease in the levels of lipid peroxidation was observed. The histopathological studies have shown the well-formed dermis with minimal inflammatory cells. Increased wound contraction, collagen migration, granulation tissue formation is attributed to the phytochemical constituents present in this plant extract. Increased levels of antioxidants favour the regeneration of wounds by quenching free radicals. Phytochemical constituents present in S. asoca may be responsible for the wound healing activity.
Deepti Bandarupalli *, Santhrani Thaakur , Srinivasa Babu Puttugunta and RLC Sasidhar
Vignan Pharmacy College, Vadlamudi, Andhra Pradesh, India
18 December, 2013
03 February, 2014
25 April, 2014
01, June 2014