SAFETY USE OF PHASEOLUS VULGARIS L. IMMATURE PODS DECOCTION AND CARDIOVASCULAR EFFECT IN RAT WITHOUT HYPERTENSION

This investigation evaluates the oral toxicity of Phaseolus vulgaris immature pods decoction a the biochemical, hematological, and cardiac profile of Wistar rats. Methods: The Economic Cooperation and Development Organization (OECD) guidelines 423 and 407 were applied. A 2000 mg/kg dose of PAD was administered to six female mice. Mortality, body weight, clinical signs of toxicity, food and water intake were recorded for 14 days. In the subacute oral toxicity test, daily doses of 100, 500, and 1000 mg/kg of PAD were administered to groups of five rats of both sexes for 28 days. Mortalities, clinical signs of toxicity, weight growth, water, and food intake were recorded throughout the period. Results: PAD did not cause mortality or significant clinical signs of toxicity at 2000 mg/kg bw. The LD₅₀ of this extract was estimated to be 5000 mg/kg bw. All animals survived daily administration of 100, 500, and 1000 mg/kg of PAD decoction. The weight gain was comparable to that of the control groups in male and female-treated rats. Furthermore, no statistically significant hydration was observed in rats given 500 and 1000 mg/kg PAD decoction compared to the rule. No alterations in the appearance, shape, size, and color of vital organs were recognized with the naked eye, magnifying glass. Furthermore, the hematologic and biochemical profiles, systolic and diastolic blood pressure and heart rate, didn’t show statistically significant variations between the control and treated groups. Conclusion: These toxicological experiments contribute to the determination of the effective preclinical dose and therapeutic range of PAD.