METHOD DEVELOPMENT AND VALIDATION USING HPLC-ESI-MS/MS FOR DETERMINING STABILITY OF TALAZOPARIB IN BIOLOGICAL MATRICES
AbstractA highly sensitive, simple, accurate and precise HPLC-ESI-MS/MS method was developed and validated for the quantitative determination of talazoparib in biological matrices. The present study describes the determination of Talazoparib using validated protein precipitation method from human plasma employing Talazoparib 13C D4 as internal standard by electron spray ionization technique. Combination of 0.1% v/v formic acid and methanol (25:75) % v/v was used as mobile phase. Ascentis Express C18 (50 mm × 4.6 mm, 2.7 µm) column was employed for the separation and the run time was 3 minutes. Flow rate was set at 0.6 ml/min and column oven temperature at 40°C for the chromatographic separation method. 10µl injection volume was selected for mass spectrometer to obtain good ionisation. Parent (Q1) and Product (Q3) ion mass transitions for Talazoparib and Talazoparib-13C-D4 were found at m/z 381.5®180.5 and 386.4®180.5 respectively. The linear regression data for the calibration plot showed a good relationship with high correlation coefficient. Correlation coefficient (r2) value from the standard curve (5-10000 pg/ml) found to be more than 0.9982.
Article Information
52
6038-6043
607 KB
275
English
IJPSR
K. Poorna Chandra Rao * and K. R. S. Sambasiva Rao
Jawaharlal Nehru Technological University, JNTUK, Kakinada, Andhra Pradesh, India.
poorna7575@gmail.com
30 April 2023
01 August 2023
04 September 2023
10.13040/IJPSR.0975-8232.14(12).6038-43
01 December 2023
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