FORMULATION, OPTIMIZATION AND EVALUATION OF RESVERATROL LOADED NANOEMULSION USING RESPONSE SURFACE METHODOLOGY

Phytochemicals are intensively addressed for clinical uses, and nanostructured phytochemical delivery vehicles are on the limelight. A viable potential treatment for neurological disorders is resveratrol. In the current investigation, we sought to create a nanoemulsion containing resveratrol utilising the response surface methodology. Using Neem oil, Pluronic-f 68, and Tween 80, the effects of the formulation and probe sonication processing parameters were analysed even during nanoemulsification process. As process factors, the duration of probe sonication has been preferred. The concentrations of oil and surfactant, along with their intensity, were revealed have a significant influence on globule size. The optimum nanoemulsion formulation constituted 2.6442 mg/ml of resveratrol. The responses picked were globule size, PDI, and zeta potential. The optimised RES-nanoemulsion had a globule size of 179 3.4 nm, a PDI of 0.175, a zeta potential of 29.9 2.1 mV, and an entrapment efficiency of RES-NEs of 96.47 2.3%. The in vitro drug release in pH 6.8 phosphate buffer displayed the nanoemulsion formulation’s superiority. The results indicate that resveratrol nanoemulsion can be blended with neem oil to improve the bioavailability of this poorly water soluble drugs. When the optimized batch has been subjected to an accelerated stability study at different temperature and relative high humidity over a three-month period, no significant variations were observed.