METHOD DEVELOPMENT, VALIDATION, AND STABILITY INDICATING STUDIES OF HYDROCHLOROTHIAZIDE IN BULK AND PHARMACEUTICAL DOSAGE FORM BY UV-SPECTROSCOPY

Development of UV spectrophotometric method for the estimation of Hydrochlorothiazide was done by Q-Absorbance ratio method and area under curve method and stability indicating studies using methanol as solvent. In the present research, we have made an attempt to develop a simple, specific, accurate, precise and reproducible method for the estimation of hydrochlorothiazide by UV spectrophotometric method, the method includes area under curve method (Method I) and Q- absorbance Ratio method (Method II). The wavelength is 272 nm λ_max of drug was selected for Method I, and for Q- absorbance Ratio method (Method II) 258 nm an iso-absorptive wavelength and 272 nm were selected for estimation of Hydro-chlorothiazide and the drug follow Beer’s law over the concentration range of 1-6 μg/ml. The % recovery of the drug was found to be nearly 100 % representing the accuracy of the proposed methods. LOD and LOQ values of hydrochlorothiazide were Found to be 0.136, 0.411, 0.181, 0.556, 0.134, 0.404 at 272 nm, 258 nm and 242 nm respectively, validation of the proposed methods was carried out for its precision, accuracy, specificity and ruggedness according to ICH guidelines. The present validated method was successfully applied for determination of hydrochlorothiazide in bulk and pharmaceutical dosage form.