DEVELOPMENT AND VALIDATION OF STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF CHLORTHALIDONE AND AMLODIPINE IN PHARMACEUTICAL DOSAGE FORM
AbstractBackground: To reduce the run and retention times of amlodipine and chlorthalidone in pharmaceutical dosage forms, a simple, affordable, time-efficient, stability-indicating, and accurate reverse-phase liquid chromatographic technique was developed. Successful chromatographic separation was achieved using Inertsil ODS-3V (250 x 4.6 mm, 5 μm particle size). A gradient consisting of water (pH 3 adjusted with formic acid) and acetonitrile was used as the mobile phase. A 20 μl injection volume was utilized, and a flow rate of 1 ml per minute was observed. The wavelength of detection was 365 nm. Method validation followed ICH norms. The medication was also found to be stable under all stress scenarios. The observed retention times for amlodipine were 1.8 minutes and chlorthalidone was 4.5 minutes. The linearity range was observed in a concentration of 50 – 150%. The percentage recovery of Chlorthalidone was 99.30% and for Amlodipine was 99.61%. The suggested technique was successfully tested and used for the estimation of amlodipine and chlorthalidone in pharmaceutical dose forms.
Article Information
43
2541-2550
758 KB
120
English
IJPSR
Abhishek D. Dhumal *, and Gayatri Barabde
Department of Chemistry, The Institute of Science, Mumbai, Maharashtra, India.
abhishekddhumal@gmail.com
29 February 2024
26 March 2024
28 March 2024
10.13040/IJPSR.0975-8232.15(8).2541-50
01 August 2024
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