REVEALING SILYMARIN: HPTLC METHOD DEVELOPMENT AND VALIDATION

Silymarin, an intricate blend of flavonolignans obtained from Silybum marianum, or milk thistle, has drawn a lot of interest because of its possible medical uses. This study reports on the development and validation of an analytical technique that employs high performance thin layer chromatography (HPTLC) to estimate silymarin in pharmaceutical dosage forms (tablet) and bulk drug. Chloroform: methanol (6:4 v/v) was the solvent system used in the chromatographic separation of the medication on aluminium plates pre-coated with silica gel 60 GF254, (10 cm × 10 cm with 250 μm layer thickness) as the stationary phase. At 288 nm, a densitometric analysis of the divided zones was carried out. Densitometric scanning was carried out at 288 nm using a Camag TLC scanner 3, which was controlled by Win CATS software. The radiation source utilised for the scan was a deuterium lamp with slit size of 5.00 x 0.45 mm. It was discovered that the retention factor was 0.42. In the 200–1200 ng/band range, linearity R2= 0.993 was used to evaluate the method’s accuracy and repeatability. Intraday and interday Precision was found 0.307- 0.544% RSD and 0.509-0.914 % RSD and accuracy was 100.146 ± 0.537%. LOD and LOQ was found 25.355ng/band, 76.833ng/band respectively, specificity and robustness in accordance with ICH guidelines.