METHOD DEVELOPMENT AND MODEST VALIDATION OF SULFADIAZINE BY REVERSE PHASE HPLC

Sulfadiazine belongs to a class of sulphonamide antibiotics. Sulfadiazine is a synthetic bacteriostatic sulphonamide that exhibits a broad spectrum of activity against gram-positive and gram-negative pathogens. The Sulfadiazine antibiotic is a member of the Sulfa drugs class. This drug is utilized for bacterial infections in the brain, ears, and urinary tract. The primary goal of the research is to develop and validate Sulfadiazine by Reverse Phase (RP) HPLC. An HPLC (Alliance, Water 2695) with a PDA detector and Avantor -ACE C18 250 mm × 4.6 mm × 5 µm column (Part Number ACE 121-2546) was used. A novel technique for the simultaneous measurement of sulfadiazine using the RP-HPLC technology was developed. The ACE C18 column (250×4.6 mm) 5µm successfully created the chromatographic conditions needed to separate sulfadiazine. The flow rate was 1 mL/min, and the mobile phase ratio of ACN: H2O was 70:30 v/v. 254 nm was the detection wavelength.