OPTIMIZED FORMULATION OF VANCOMYCIN LOADED THERMOREVERSIBLE HYDROGEL FOR TREATMENT OF ORTHOPEDIC INFECTIONS
AbstractThe aim of the present study was to develop suitable controlled release injectable vancomycin thermoreversible chitosan/ β-glycerophosphate hydrogel system for treatment of orthopedic infections. The effect of different formulation variables on the gelation temperature was studied. The prepared vancomycin loaded Chitosan/β-glycerophosphate thermoreversible hydrogels were characterized regarding gelation temperature, in-vitro vancomycin release and enzymatic degradation rate in presence of lysozyme. Antimicrobial activity of the released vancomycin was tested against the gram-positive methicillin resistant Staphylococcus aureus (MRSA) by agar diffusion method. The results showed that chitosan/ β-glycerophosphate solution were able to form gels at body temperature depending on chitosan degree of deacetylation, concentration, pH and β-GP concentration. The prepared thermoreversible hydrogels sustained vancomycin release for 21 days. Released vancomycin concentrations ranged from 3 to 95 folds the reported minimal inhibition concentration (MIC 90) for MRSA at all release study time points. Chitosan/β-glycerophosphate thermoreversible hydrogels were enzymatically degradable and 17.57% of the gel weight remained after 21 days. The preserved antimicrobial activity found after release of the antibiotic from the hydrogel elected this delivery system for further in-vivo and toxicological investigations.
Article Information
40
2936-2946
995
1648
English
IJPSR
Hend Mohamed Abdel-Bar, Amal Youssef Abdel-Reheem, Rihab Osman*, Gehanne A.S. Awad and Nahed Mortada
Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt
rihabosman@pharma.asu.edu.eg
01 February, 2014
01 February, 2014
24 March, 2014
http://dx.doi.org/10.13040/IJPSR.0975-8232.5(7).2936-46
01 July, 2014