BIOSYNTHESIS, PURIFICATION, AND CHARACTERIZATION OF MONK FRUIT SWEETENER, SIAMENOSIDE I
AbstractAn efficient and high yield synthesis of Siamenoside I (1), from Mogroside V (2) is reported. Siamenoside I (1) was obtained by enzymatic hydrolysis of Mogroside V (2). Bioconversion process involved enzymatic modification followed by separation and purification processes, including reversed-phase chromatography. The final product, a dried white powder, undergoes several stages of concentration, reaction, filtration, and freeze-drying. Siamenoside I (1) was isolated and characterized with NMR (1H, 13C, 1H-1H COSY, 1H-13C HSQC-DEPT, 1H-13C HMBC, 1H-13C HSQC-TOCSY, 1H-1H ROESY and 1D-TOCSY) and mass spectral data. This Siamenoside I (1) possess a 1->2 beta sugar linkage between sugar II and sugar I, making it’s structural properties unique. The sensory and spectral properties of Siamenoside I (1) synthesized from mogroside V (2) or isolated from fruit have the same characteristics. Additionally, we emphasize the importance of 1D and 2D NMR techniques when identifying complex mogrosides.
Article Information
13
2250-2258
718 KB
23
English
IJPSR
Indra Prakash *, Christopher P. Mercogliano, Gil Ma and Steven F. Sukits
The Coca-Cola Company, Atlanta, GA, 30313, USA.
iprakash@coca-cola.com
08 March 2025
16 April 2025
25 April 2025
10.13040/IJPSR.0975-8232.16(8).2250-58
01 August 2025
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