SIMULTANEOUS ESTIMATION AND QUANTIFICATION OF ROSUVASTATIN AND FENOFIBRATE USING HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY (HPTLC) IN PHARMACEUTICAL DOSAGE FORM
AbstractA sensitive, specific and precise HPTLC method for simultaneous estimation of Fenofibrate and Rosuvastatin has been developed and validated. The method employed aluminium plates pre-coated with silica gel 60 F254 as the stationary phase. The solvent system consisted of n-Hexane: Acetone: Ethyl acetate: Glacial acetic acid (4: 5: 0.9: 0.1, v/v/v/v) which was found to give compact and dense spots for Fenofibrate and Rosuvastatin (RF value 0.83 ± 0.05 and 0.35 ± 0.05 respectively). Densitometric analysis of both drugs was carried out at 300 nm. Linear relationship was found with calibration curve for linearity in concentration range of 25-75 mg/spot and 167-501 mg/spot for Rosuvastatin and Fenofibrate with Correlation coefficient value 0.992 and 0.991 respectively. This method was found to be good percentage recovery for Rosuvastatin and Fenofibrate in between 98% to 102%. All parameters of validation of the formulations were found within the limit as per ICH guidelines.
Article Information
23
2368-2374
702 KB
37
English
IJPSR
Shailendra V. Dalvi *, Gurunath V. Pandit, Prashant J. Lokhande and Sharada Jadhav
Quantitative Quality Chemistry, Chikitsak Samuha’s Patkar-Varde College, Goregaon, Mumbai, Maharashtra, India.
ggundipatkarvardecollege@gmail.com
15 March 2025
29 March 2025
15 April 2025
10.13040/IJPSR.0975-8232.16(8).2368-74
01 August 2025
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