DETERMINATION OF IN-VITRO METABOLIC STABILITY OF OLEANOLIC ACID DERIVED FROM LANTANA CAMARA ROOTS USING MOUSE, RAT, AND MONKEY LIVER MICROSOMES

The objective of the present study was to assess the in-vitro metabolic stability of oleanolic acid isolated from Lantana camara roots using liver microsomes from mouse, rat, and monkey, and to investigate interspecies differences in metabolic behaviour. Oleanolic acid was incubated with pooled liver microsomes in the presence of NADPH at 37 °C, and the depletion of the parent compound over time was quantified using an automated LC–MS/MS platform. Metabolic half-life (t½) and intrinsic clearance (CLint) were calculated based on the rate of disappearance of oleanolic acid during incubation. The results demonstrated clear species-dependent differences in metabolic stability. Oleanolic acid showed the longest half-life and lowest intrinsic clearance in monkey liver microsomes, indicating higher metabolic stability, followed by moderate stability in rat microsomes. In contrast, mouse liver microsomes exhibited a shorter half-life and higher intrinsic clearance, suggesting faster metabolic turnover. These findings reflect significant interspecies variability in microsomal metabolism of oleanolic acid. In conclusion, oleanolic acid exhibited moderate to good in-vitro metabolic stability, particularly in higher species, supporting its potential for further pharmacokinetic evaluation.