DEVELOPMENT AND VALIDATION OF A REVERSED PHASE HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF OLMESARTAN MEDOXOMIL AND HYDROCHLOROTHIAZIDE IN COMBINED TABLET DOSAGE FORM
AbstractA simple reversed phase HPLC method has been developed for the simultaneous determination of olmesartan medoxomil in combination with hydrochlorothiazide. The method was based on reversed phase liquid chromatography using a Grace Smart RP C18 column (250 × 4.6 mm, 5µ) with UV detection at 256 nm. The mobile phase consisting of acetonitrile and triethylamine buffer adjusted to pH 2.7 in a ratio of (30:70, v/v) and at a flow rate of 2 ml/min. The method was linear over the concentration range for olmesartan medoxomil 4.0-40.0 μg/mL and for hydrochlorothiazide 5.0-50.0 μg/mL. The recoveries of olmesartan medoxomil and hydrochlorothiazide were found to be in the range of 99.25-100.46% and 99.31-102.13% respectively. The method was validated and was successfully employed for the analysis of pharmaceutical formulations containing olmesartan medoxomil and hydrochlorothiazide in combined tablet dosage form.
Article Information
9
80-84
410 kB
1369
English
IJPSR
Moynul Hasan*, Abdullah Al Masud and Jamiuddin Ahmed
Department of Pharmacy, Dhaka International University, Banani, Dhaka, Bangladesh
19 July, 2010
07 October, 2010
01 November, 2010
http://dx.doi.org/10.13040/IJPSR.0975-8232.1(12).80-84
01 December, 2010
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