ESTIMATION OF TEICOPLANIN IN PARENTERALS BY RP-HPLC
AbstractA simple, precise, rapid and accurate Reverse Phase HPLC method was developed for the estimation of Teicoplanin in Parenteral dosage form. An Xterra RP C18, 250×4.6 mm, column with 5 µm particle size and the mobile phase consisting of 0.02M Potassium Dihydrogen Orthophosphate + 0.02M Dipotassium Hydrogen Orthophosphate + 0.5% Triethyl Amine in water pH: 3.2 adjusted with Orthophosphoric Acid: Acetonitrile (40:50). Acetonitrile in the isocratic mode was used. The flow rate was 0.5 ml/min and the effluents were monitored at 210 nm. The retention time was 4.178 min. The detector response was linear in the concentration of 24.1-289.2 mcg/mL for Teicoplanin, the respective linear regression equation being Y (-242937.9235) = 71026.949x + 174544.8363 for Teicoplanin. The Limit of Detection (LOD) and The Limit of Quantification (LOQ) were 1.205 mcg/mL and 3.615 mcg/mL respectively for Teicoplanin. The percentage assay of Teicoplanin was 99.64 %. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Teicoplanin in bulk drug and in its pharmaceutical dosage forms.
Article Information
34
1807-1810
556
1164
English
Ijpsr
Ravi Pratap Pulla, B. S. Sastry , Y. Rajendra Prasad and N. Appala Raju
Department of Pharmaceutical Chemistry, SSJ College of Pharmacy, V.N.Pally, Gandipet, Hyderabad-500 075, Andhra Pradesh, India
13 April, 2011
21 May, 2011
26 June, 2011
http://dx.doi.org/10.13040/IJPSR.0975-8232.2(7).1807-10
01 July, 2011
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