VALIDATED RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF PARACETAMOL, PAMABROM AND DICYCLOMINE, HYDROCHLORIDE IN BULK AND PHARMACEUTICAL DOSAGE FORM

Simple and rapid Reverse Phase High-Performance Liquid Chromatography (RP-HPLC)  and UV-spectrophotometry method was developed for the determination of Paracetamol, Pamabrom and Dicyclomine hydrochloride, detection was carried out using photo diode array detector. Chromatographic separation of the analytes was achieved within 3.12, 4.25, 5.35 min for Paracetamol, Pamabrom and Dicyclomine hydrochloride by LC-GC Qualisil Gold-C18  (250 x 4.6 mm i.d., 5µm) column, mobile phase was methanol : water (1% TFA with pH adjusted to 3.0 with ammonia) in the ratio of (83:17), 0.4% (v/v) TEA and was filtered and degassed., flow rate was 1.0 mL/min, and the detection was carried out at 221 nm. Calibration curve was linear (r2=0.9993) in the range of 13-78 g/mL for Paracetamol, r2=0.9991 in the range of 1-6 g/mL for Pamabrom and r2=0.9993 in the range of 250-1500 g/mL for Dicyclomine hydrochloride. The commonly used excipients and additives present in the pharmaceutical formulations were free from interfering components, as illustrated by Specificity of the method. Accuracy was performed as recovery study and results showed the recovery value of pure drug from the solution, between 98-102%, which indicates the method is accurate. Hence this method was better for Pharmaceutical formulations analysis