DEVELOPMENT OF VALIDATED RP-HPLC METHOD FOR QUANTITATIVE ESTIMATION OF RIVASTIGMINE HYDROGEN TARTRATE IN TRANSDERMAL DRUG DELIVERY SYSTEM
AbstractA fast simple sensitive precise, accurate and reproducible RP-HPLC method was developed and validated for the analysis of rivastigmine hydrogen tartrate in transdermal drug delivery system. Rivastigmine tartrate is soluble in water so it was used as solvent. The separation was conducted by using C-18 RP-HPLC column which was maintained at ambient temperature.The mobile phase consist 0.01M ammonium acetate buffer and acetonitrile (70:30 v/v) was delivered at a rate of 1ml/min. The analysis was detected by using UV detector at the wavelength 219nm. The method is validated for its accuracy precision,ruggedness, linearity and range. The method was found to be linear over the concentration range 50-100 µg/ml (r2 = 0.999). The retention time for rivastigmine was found to be 4.40min.The total run time of chromatogram was about 10min.The % R.S.D. value less than 2 indicate that the method is precise. Ruggedness of the proposed method was studied with the help of two analysts. The above method was a rapid and cost-effective quality-control tool for routine analysis of Rivastigmine hydrogen tartrate in transdermal drug delivery system.
Article Information
35
1892-1902
419KB
2655
English
IJPSR
M. N. Kale
Sudhakarrao Naik Institute of Pharmacy college, Nagpur road, Pusad-445204, Dist. Yavatmal, Maharashtra, India
kalemeenan@gmail.com
05 December, 2013
06 February, 2014
16 March, 2014
http://dx.doi.org/10.13040/IJPSR.0975-8232.5(5).1892-02
01, May 2014
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