GREEN SYNTHESIS OF SILVER NANOPARTICLES USING TABERNAEMONTANA DIVARICATA AND IN-VITRO CYTOTOXICITY INVESTIGATION AGAINST HUMAN LUNG ADENOCARCINOMA

The present study aims to synthesize highly stable and dispersed cytotoxic silver nanoparticles (AgNPs) from Tabernaemontana divaricata (T. divaricata) leaves extract, by using noble green approach at room temperature. Phytocomponents were investigated using general methods and found good agreement with reported literature. Green synthesized AgNPs were characterized using UV-Vis, FT-IR, XRD, TGA, FEG-SEM, EDX, TEM, SAED and AFM spectroscopy techniques. XRD and SEAD confirmed polycrystalline nature. TEM study shows spherical morphology with size range 6-20 nm. Suforhodamine B (SRB) assay revealed potent cytotoxicity of AgNPs on A549 cells with IC₅₀ value 32.3µg/ml. As per SRB assay in case of AgNPs, GI₅₀ = 37.1µg/ml, TGI = 68.9µg/ml, LC₅₀ = > 80µg/ml, while T. divaricata exhibits GI₅₀, TGI, LC₅₀ values = > 80µg/ml, indicating greater biocompatibility of AgNPs in comparison to T. divaricata leaves extract. This investigation reveals that T. divaricata mediated AgNPs has a great potential for the development of an efficient anticancer nano drug with minimal side effects to combat against life-threatening lung cancer.