MATHEMATICALLY DESIGNED BIOPROCESS FOR RELEASE OF VALUE ADDED PRODUCTS WITH PHARMACEUTICAL APPLICATIONS FROM WASTES GENERATED FROM SPICES INDUSTRIES

A mathematical model optimizing bio-physico-chemical pre-treatment of steam exploded Piper nigrum, and Syzygium aromaticum lignocellulosic biomass waste was developed. The model was developed using, RSM, involving central composite face-centered design (CCD) with six parameters, three levels and 40 runs, at 95% confidence level. Six parameters optimized were quantity of each enzyme (a) Cellulase (ml);  (b) Xylanase (ml) for enzymatic actions at 50 ºC; (c) Incubation time of 2-15 days for which substrate were dipped in various solvent systems; (d) Volume of solvent systems used; (e) Time of incubation at given steam pressure for steam explosion; and (f) Steam pressure 10-15 Psi. Cellulase and xylanase enzymes used were with activity (3.563 IU/mL), (33.32 IU/mL) respectively. ANOVA was applied for validation of the predicted model at 95% of confidence level. The Base 10 log transformation was selected from the comparative study, and the model predicted 13.79 µmoles/mL, the release of polyphenols at 2 days of incubation of substrate with 10 ml of the solvent system, under 15.0 psi steam explosion pressure for 15 min, with the treatment of 1.00 ml of cellulose. The released polyphenols were tested against human pathogenic microbes (Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Micrococcus) and found to possess strong antimicrobial properties.