EVALUATION OF α-AMYLASE AND α-GLUCOSIDASE ENZYME INHIBITORY ACTIVITIES OF TRICHURIELLA MONSONIAE BENNET

Diabetes is a noncommunicable chronic metabolic disorder characterized by high blood glucose level in the body. One of the antidiabetic therapeutic strategies is the inhibition of carbohydrate-digesting enzymes such as α-amylase and α-glucosidase. In the present study methanolic extract and its two fractions (Ethyl acetate and n-butanol) of the whole plant of Trichuriella monsoniae were evaluated for their effect on α-amylase and α-glucosidase enzymes by using in-vitro assays. The plant extracts were used at varying concentrations to ensure which concentration of the extract/fractions of the plant causes the most inhibition. Phytochemical analysis of the extract and its fractions indicated the presence of flavonoids, steroids/terpenoids, carbohydrates, saponin, phenolic compounds. While tannins and alkaloids are absent.  n-butanol fraction shown the prominent α-amylase and α-glucosidase enzyme inhibitory activities (IC₅₀ 4.09 mg/ml and 3.30 mg/ml respectively) than methanolic extract and its ethyl acetate fraction, and it was well comparable with the standard drug acarbose (for α-amylase IC₅₀ 3.62 mg/ml and for α-glucosidase IC₅₀ 2.19 mg/ml). Further, the Total phenolic and flavonoid contents were estimated. These results suggest that Trichuriella monsoniae with a great potential to control postprandial hyperglycemia might be a novel resource for the management of type 2 diabetes.