THE IN-VITRO ANTI-DENATURATION EFFECTS INDUCED BY SUBSTITUTED OXY/THIOXY PYRIMIDINE DERIVATIVES IN BOVINE SERUM ALBUMIN IS PROPOSED AS A SCREENING ASSAY FOR THE DETECTION OF ANTI-INFLAMMATORY COMPOUNDS WITHOUT THE USE OF ANIMALS
AbstractA series of novel 2-{[6-(2-substituted Phenyl)-2-oxo-1, 2-dihydropyrimidin -4-yl]oxy} benzoic acid (2a-b)/ 2-{[6-(2-substituted Phenyl)-2 thioxo-1, 2-dihydropyrimidin-4-yl]oxy} benzoic acid (2c-d) were synthesized and tested for their in-vitro protein denaturation activity. Compound 2d was found to be promising and was more potent than the Acetylsalicylic acid (NSAID) in the inhibition of Bovine serum albumin denaturation. The purity of the compounds was checked by TLC and elemental analyses. Both analytical and spectral data (1H NMR, IR, and MS) of all the synthesized compounds were in full agreement with the proposed structures.
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