ISOLATION AND CYTOTOXIC POTENTIAL OF SILVER NANOSUSPENSION OF THE ROOTS OF APHANAMIXIS POLYSTACHYA

Objective: The study was aimed to isolate the components from the methanolic extract of the root bark of Aphanamixis polystachya and to confirm their potential cytotoxicity of the methanolic extract, silver nanoparticles and silver nanosuspension of the root bark of A. polystachya. Method: Isolation from the methanolic extract of root barks of A. polystachya were performed using column chromatography technique. Cytotoxic activity on MCF-7 and MDA-MB cell lines by SRB Assay was conducted on silver nanosuspension of A. polystachya. Results: The components isolated from methanolic extract of root bark of A. Polystachya by column chromatography were confirmed as Rohituka 7, Rohituka 3, Amoorinin-3-o-α-l-Rhamnopyranosyl-(1à6)–β-D-Glucopyra-noside and 8- Methyl-7, 2׳,4׳-tri-O-Methylflavonone-5-O-α-L-Rhamnopyranosyl (1 4)-β-D- glucopyranosyl-(1 6)-β-D-glucopyranoside on the basis of IR, ¹HNMR, ¹³C NMR and MS. The prepared silver nanosuspension, tested in concentrations ranging between 500-7.81 µM showed IC₅₀ value at 0.58 µM in MCF-7 cell line, and IC₅₀ in MDA-MB cell line being greater than 1000µM. Conclusion: Column chromatography of the methanolic extract of root barks of A. polystachya led to the isolation of Rohituka 7, Rohituka 3, Amoorinin-3-O-α-L-Rhamnopyranosyl-(1à6)–β-D Glucopyranoside and (methyl-7,2׳,4׳-tri-O-methyl flavonone-5-O-α-L-rhamnopyranosyl(1à4)-β-D-glucopyranosyl-(1à6)-β-D-glucopyranoside. SRB Assay confirmed that silver nanosuspension of A. polystachya to be potentially cytotoxic. The isolated components have already proven to possess cytotoxic activity. Hence, the study suggests that silver nanosuspension exhibited better cytotoxic activity.