STANDARDIZATION OF AYURVEDIC FORMULATION “ARJUNARISHTA” IN TERMS OF PHYSICOCHEMICAL, SPECTROSCOPY AND CHROMATOGRAPHIC TECHNIQUES

Objective: Arjunarishta is an important Ayurvedic formulation with chief ingredient Arjuna (Terminalia arjuna), which is excellent “Hrudya” Dravya. It nourishes & strengthens heart muscles & promotes cardiac functioning by regulating blood pressure and cholesterol level. This study aims to established quality parameters for standardization of Ayurvedic formulation “Arjunarishta” on the basis of various techniques viz. Physico-chemical screening, Chromatography & IR Spectroscopy. Method: Conventional analysis such as Organoleptic tests, pH, Alcohol content, Brix, and Specific gravity was done. Chromatographic analysis was performed to estimate Arjungenin, Ellagic acid, and Gallic acid using High-Performance Thin Layer Chromatography (HPTLC). IR fingerprint was done using Fourier transform infrared (FT-IR) spectrometer. Result: The above analysis showed the pH of tested formulation ranges between 3.0-5.0, Brix 25-30%, and Alcohol content 6-10% v/v. Organoleptic tests confirmed characteristic odor of self-generated alcohol with sweet, astringent & slightly bitter taste. HPTLC analysis confirmed the presence of Arjungenin, Ellagic acid, and Gallic acid. FT-IR reveals unique transmittance spectra in the range of 4000 – 600 cm^-1. Conclusion: This study can be used for qualitative evaluation of Arjunarishta in terms of modern parameters, which may help in the authenticity of the drug and to compile suitable information for the better utility and safe use of this formulation in therapeutics.