UV-SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF HYDROXYCHLOROQUINE SULFATE AND NITAZOXANIDE IN SYNTHETIC MIXTURE
AbstractA simple, accurate, precise, reproducible, economical UV spectrophotometric method has been developed and validated for simultaneous estimation of Hydroxychloroquine sulfate (HCQ) and Nitazoxanide (NTZ) in a synthetic mixture. Method A by simultaneous equation method, Method B by the first-order derivative method. Method A: simultaneous equation method was based on the measurement of absorbance of nitazoxanide at 345 nm and Hydroxychloroquine Sulfate at 220 nm. Method B: first-order derivative method was based on the measurement of absorbance of HCQ measure at 226 nm (ZCP of NTZ) and absorbance of NTZ measure at 375.50 nm (ZCP of HCQ). The calibration curve was found to be linear in the concentration range 2-10 μg/ml and 5-25μg/ml for HCQ and NTZ, respectively (n=6), with their correlation coefficient of 0.999. The developed method was validated according to the ICH guideline.
Article Information
41
1374-1382
832 KB
587
English
IJPSR
Mahyavanshi S. Aayushi * and Jasmina S. Surati
Department of Pharmaceutical Quality Assurance, Shree Lal Bhai Patel college of Pharmacy, Bardoli, Gujarat, India.
aayushimahyavanshi153@gmail.com
16 June 2021
24 July 2021
26 July 2021
10.13040/IJPSR.0975-8232.13(3).1374-82
01 March 2022
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