EFFECT OF SUGARCANE BAGASSE AS A SOURCE OF CARBON FOR ɑ-AMYLASE ENZYME AND ANTIBACTERIAL COMPOUND PRODUCING DIFFERENT TYPES OF WILD FILAMENTOUS FUNGI

The present investigation screening and characterization of α-amylase and antimicrobial compound producing filamentous fungi is used for extensive commercial applications in various sectors and is highly demanded in the Biotech industry. The results reveal that out of the carbon substrates alone, sugarcane bagasse with a combination of Tween-80 was found to yield maximum α-amylase (151.76 IU) under solid-state fermentation by different species of fungal species such as KRV_001Chaetomium oryzae, KRV_002 Penicillium citrinum, KRV_003 Aspergillus flavus, KRV_004 Penicillium notatum, KRV_005 Alternaria longipes and KRV_006 Trichoderma polysporum. Among them, KRV_001 Chaetomium oryzae, KRV_002 Penicillium citrinum, KRV_005 Alternaria longipes were maximum expressed α-amylase based on the plate assay by different pH, temperature and different substrate using amylase activity, SDS-PAGE, Antimicrobial activity has been done in Methanol extract exhibited antibacterial activity against S. aureus MTCC96 whereas the water extract displayed antibacterial activity. The organism produces volatile and water-soluble antibiotics and shows significant inhibition as the potential value of KRV_001Chaetomium oryzae and little less activity of KRV_006 Trichoderma polysporum among the isolates was investigated.