A MOLECULAR MODELING STUDY OF PHYTOCONSTITUENTS ANALYSED VIA GC-MS TECHNIQUE IN THE RHIZOME PART OF HEDYCHIUM SPICATUM (ZINGIBERACEAE)

Hedychium spicatum is one of the plant species reported from the northern Himalayan ranges of India, belonging to the family Zingiberaceae, with ethnobotanical values and is well-known for their ethnomedicinal applications. In the present investigation, pet ether and ethanolic rhizome extracts of Hedychium spicatum were Analyzed by gas chromatography-mass spectrometry (GC–MS) to identify the important phytochemical constituents. The GC–MS analysis of pet ether and ethanolic extracts from rhizomes of Hedychium spicatum detected the presence of numerous phytochemical compounds. Further, the literature review reported rhizome extracts of H. spicatum to have remarkable anti-inflammatory, antioxidant, cardioprotective, and antibacterial activities. The results of DPPH and ferric reducing antioxidant power assay recorded maximum antioxidant activity in Hedychium spicatum ethanolic rhizome extract at 500mg/kg dosage. As well as the ethanolic extract exhibited maximum α-amylase inhibition activity followed by exhibiting aldose reductase inhibition. Subsequently, the six identified compounds were analyzed for their bioactivity through in-silico molecular docking studies. Results revealed that 7-hydroxyhedychenone might have maximum antioxidant and antidiabetic properties among the phytochemical compounds identified, followed by spicatanol and spicatanol methyl ether. To our best knowledge, this is the first description of some of the phytochemical constituents of rhizomes of H. spicatum, which show pharmacological significance, as there has been no literature available yet on in-silico molecular docking of 7-hydroxyhedychenone was also performed to confirm its pharmacological antidiabetic activity based on the binding interactions with the aldose reductase target proteins. The present study results will create an understanding of the antidiabetic pharmacological mechanism of action, which may lead to the development of novel drugs.