A NEW, SIMPLE, SENSITIVE, ACCURATE & RAPID ANALYTICAL METHOD DEVELOPMENT & VALIDATION FOR SIMULTANEOUS ESTIMATION OF LAMIVUDINE, ABACAVIR & ZIDOVUDINE IN TABLET DOSAGE FORM BY USING UPLCAbstract
The present work was undertaken to develop and validate a rapid and consistent UPLC method in which the peaks will appear in a short period as per ICH Guidelines. The UPLC separation was achieved on a Symmetry C18 (2.1 × 100mm, 1.7mm, Make: BEH) or equivalent in an Isocratic Mode. The mobile phase was composed of Phosphate Buffer (60%) [pH 3.0] & Methanol (40%) [UPLC Grade] The flow rate was monitored at 0.25 ml per min. The wavelength was selected for the detection was 280 nm. The run time was 3 min. The retention time found for the drugs Lamivudine, Abacavir, and Zidovudine was 1.019 min, 1.271 min & 1.617 min respectively. The % recovery was found to be 98.0%- 99.0% for the drug Abacavir. The % recovery was found to be 98.0% – 99.6% for the drug Lamivudine. The % recovery was found to be 98.2% – 98.6% for the drug Zidovudine. The linearity was established in the range of 20 to 60 ppm for the drug Abacavir & 10 to 30 ppm for the drug Lamivudine & 20 to 60 ppm for the drug Zidovudine. The LOD for the drugs Abacavir, Lamivudine, and Zidovudine were found to be 0.002 µg/ml, 0.003 µg/ml, & 0.005 µg/ml, respectively. The LOQ for the drugs Abacavir, Lamivudine, and Zidovudine were found to be 0.008 µg/ml, 0.01 µg/ml & 0.02 µg/ml respectively. Overall the proposed method was found to be suitable, sensitive, reproducible, specific and accurate for the quantitative determination of the drug in tablet dosage form.
G. S. K. Reddy *, S. A. Kumar and V. R. Kumar
Department of Pharmaceutical Analysis & Quality Assurance, Sana College of Pharmacy, Kodad, Nalgonda, Andhra Pradesh, India.
03 March 2014
08 May, 2014
26 June 2014
01 September 2014