A NOVEL STABILITY INDICATING RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF MOXIFLOXACIN AND PREDNISOLONE IN BULK AND THEIR COMBINED DOSAGE FORMAbstract
A simple, specific, and precise stability indicating reverse phase high performance liquid chromatography method was developed and validated as per the ICH guidelines for the simultaneous determination of Moxifloxacin and Prednisolone in bulk and combined dosage forms. The quantification was carried out by using Zodiac C18 (250mm*4.6mm, 5μ) column at 250c with Acetate Buffer pH 4.5: Methanol: Acetonitrile in ratio of 50:20:30 % V/V as mobile phase. The flow rate is 1 mL/min and the estimation was carried out by using PDA detector at 271 nm. The retention time of MFX and PDS were 2.317 and 4.310 minutes respectively. The linearity was observed from 30-70μg/mL with correlation coefficient 0.9999 for Moxifloxacin and 60-140 μg/mL with correlation coefficient 0.9998 for Prednisolone. The LOD and LOQ of Moxifloxacin and Prednisolone were found to be 4.85 & 14.69μg/mL and 9.07 and 27.47μg/mL respectively and the Statistics data for the MFX and PDS were concluded that the method was found to be simple, reliable, selective, reproducible and accurate. The method was successfully used for quality control analysis of Moxifloxacin and Prednisolone
Naga Raju Potnuri*, Devala Rao G and Rajendra Prasad Y
Associate Professor Joginpally B.R. Pharmacy College, Yenkapally (V), Moinabad (M), R.R. (Dist.), A.P, India.
10 September, 2014
13 November, 2014
06 January, 2015
01 May, 2015