A RP-HPLC METHOD DEVELOPMENT FOR THE ESTIMATION OF RIZATRIPTAN BENZOATE FROM FORMULATED FAST DISINTEGRATING SUBLINGUAL TABLETSAbstract
A simple, accurate, precise RP-HPLC method has been developed for the estimation of Rizatriptan Benzoate. Good chromatographic separation was achieved by isocratic mode with Sodium dihydrogen orthophosphate as buffer (pH 3.5): Acetonitrile (80:20), as the mobile phase with Zorbax SB phenyl column (250 × 4.6 mm i.d, 5 µ particle size) as stationary phase with a flow rate of 1.0 ml/min at ambient temperature. Quantification was attained with UV detection at a wavelength of 255 nm. The retention time for Benzoate and Rizatriptan was 3.85 minutes and 4.32 minutes, respectively. The calibration graphs were linear in the concentration range of 5 – 25 μg/ml. The developed method was effectively applied to formulated fast disintegrated sublingual tablets of Rizatriptan Benzoate, and the % assay of the drug was found to be 96.4%. This method was statistically validated for the determination of accuracy, precision, specificity, linearity, range, the limit of detection, the limit of quantification and system suitability according to ICH guidelines.
B. Haarika * and V. P. Reddy
S. N. Vanitha Pharmacy Maha Vidyalaya, Exhibition grounds, Hyderabad, Andhra Pradesh, India.
06 March 2014
29 April 2014
30 June 2014
01 September 2014