A SENSITIVE RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF DIETHYLCARBAMAZINE AND LEVO-CETIRIZINE IN BULK AND PHARMACEUTICAL DOSAGE FORMAbstract
A simple, sensitive and reproducible method was developed and validated for the simultaneous estimation of Diethylcarbamazine and Levocetirizine in its tablet formulation by reverse phase high performance liquid chromatography using Waters1515 HPLC with Diethylcarbamazine and Levocetirizine simultaneously. HPLC-PDA detector at the λmax of 253nm, using Hypersil-BDS C18 (250×4.6 mm. 5 μ) column. The mobile phase used was potassium dihydrogen orthophosphate buffer (pH: 5): acetonitrile (20:80 v/v) with isocratic flow (flow rate 1 ml/min) and the pH was adjusted with orthophosphoric acid. The compounds Diethylcarbamazine, Levocetirizine were eluted at 2.04, 5.54 min, respectively. The peaks were eluted with better resolution. The method was accurate with assay values of 99.67% and 99.81% w/w, precise (%RSD) with 0.3and 0.5, percentage recovery values of pure drug were in between 99.4% to 100% and 99% to 99.4% which are very sensitive with limit of detections (LOD)’s 2.42 and 0.08ppm and limit of quantification (LOQ)’s 7.42 and 0.2ppm, these results are within the range of limits 98% to 101% which indicates that the method was accurate and linear with R2 values 0.999 in the range of 20to 120 μg/ml 0.8 to 4.8 μg/ml for Diethylcarbamazine and Levocetirizine, respectively.
Krishna Vamsi Movva*, B. Jayalakshmi, R. Vijay Amritharaj and Sandeep Chilamkurthi
Department of Pharmaceutical Analysis, JKK Munirajah Medical Research Foundation College of Pharmacy, Komarapalayam-638183, Namakkal, Tamil Nadu, India
07 June, 2012
06 July, 2012
27 August, 2012
01 September, 2012