A SENSITIVE RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF DIETHYLCARBAMAZINE AND LEVO-CETIRIZINE IN BULK AND PHARMACEUTICAL DOSAGE FORM
HTML Full TextA SENSITIVE RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF DIETHYLCARBAMAZINE AND LEVO-CETIRIZINE IN BULK AND PHARMACEUTICAL DOSAGE FORM
Krishna Vamsi Movva*, B. Jayalakshmi, R. Vijay Amritharaj and Sandeep Chilamkurthi
Department of Pharmaceutical Analysis, JKK Munirajah Medical Research Foundation College of Pharmacy, Komarapalayam-638183, Namakkal, Tamil Nadu, India
ABSTRACT
A simple, sensitive and reproducible method was developed and validated for the simultaneous estimation of Diethylcarbamazine and Levocetirizine in its tablet formulation by reverse phase high performance liquid chromatography using Waters1515 HPLC with Diethylcarbamazine and Levocetirizine simultaneously. HPLC-PDA detector at the λmax of 253nm, using Hypersil-BDS C18 (250×4.6 mm. 5 μ) column. The mobile phase used was potassium dihydrogen orthophosphate buffer (pH: 5): acetonitrile (20:80 v/v) with isocratic flow (flow rate 1 ml/min) and the pH was adjusted with orthophosphoric acid. The compounds Diethylcarbamazine, Levocetirizine were eluted at 2.04, 5.54 min, respectively. The peaks were eluted with better resolution. The method was accurate with assay values of 99.67% and 99.81% w/w, precise (%RSD) with 0.3and 0.5, percentage recovery values of pure drug were in between 99.4% to 100% and 99% to 99.4% which are very sensitive with limit of detections (LOD)'s 2.42 and 0.08ppm and limit of quantification (LOQ)'s 7.42 and 0.2ppm, these results are within the range of limits 98% to 101% which indicates that the method was accurate and linear with R2 values 0.999 in the range of 20to 120 μg/ml 0.8 to 4.8 μg/ml for Diethylcarbamazine and Levocetirizine, respectively.
Keywords:C18 column,
Diethylcarbamazine (DEC), Levocetirizine (LEVC), |
RP-HPLC, validation
INTRODUCTION: Diethylcarbamazine (DEC) is a piperazine anthelmintic agent indicated for the treatment of individual patients with lymphatic filariasis, tropical pulmonary eosinophilia and loiasis. The chemical name of the drug is N, N-diethyl-4-methylpiperazine-1-carboxamide citrate]. It acts by inhibiting arachidonic acid metabolism and it is a polar compound.
Levocetirizine (LEVC) is a third generation non-sedative antihistamine developed from second-generation antihistamine; cetirizine. Chemically LEVC is active enantiomer of cetirizine. The chemical name is 2-(2-(4-((R)-(4-chlorophenyl)-phenyl-methyl) piperazin-1-yl) ethoxy) acetic acid dihydrochloride.
It is more effective with fewer side effects than second generation drugs. It works by blocking histamine receptors and it is polar compound in nature. Both drugs have good pharmacological actions. Many formulations are marketed individually or combination with other drugs.
UV/Vis spectrophotometric methods, High Performance Liquid Chromatographic (HPLC) methods, liquid chromatography-tandem mass spectroscopic (LC-MS/ MS) method and gas chromatographic (GC) method are available for estimation of DEC and LEVC in formulations individually or in combination with other compounds or in plasma samples.
DEC and LEVC combined formulation is recently available marketed product. Literature survey showed that no HPLC method is available for estimation of these drugs simultaneously. The present study aims in developing RP-HPLC method for simultaneous estimation of these compounds in formulations.
EXPERIMENTAL
Materials: All the chemicals used were of HPLC grade. Potassium dihydrogen orthophosphate was obtained from Qualigens fine chemicals, orthophosphoric acid and acetonitrile were obtained from Rankem Fine Chemicals, Mumbai, India. All the drugs DEC citrate, LEVC dihydrochloride and losartan potassium (IS) were purchased from Sigma Aldrich chemicals, Bangalore, India. Tablets of Levodec (equivalent to 150 mg of DEC and 2.5 mg of LEVC, Reign (India) Formulations Pvt. Ltd, and Mettupalayam, India) were purchased from a local pharmacy. The ultra pure water used was collected from Millipore system.
- Procedure: The method development was performed with Waters 1515 HPLC system (Dual λmax 2487 UV detector), Rheodyne 7725i injector with 20-μl loop and the output signal was monitored and integrated using Breeze software (3.30 version), Shimadzu UV 1700 spectrophoto-meter for optimizing the wave length. Sartorious digital balance, Systronics pH meter and ultra sonicator were used. Shimadzu Prominence HPLC (LC-20AT pump, SPD-20A detector) was used for determining the ruggedness of the method. Mobile phase used was potassium dihydrogen orthophosphate buffer adjusted to pH 5 and acetonitrile with 20:80% v/v, it was filtered and degassed by ultra sonication.
Preparation of Standard: Standard solutions of DEC, LEVC were prepared separately at a concentration of 1 mg/ml and further dilutions were made to prepare working standard solutions used for validation studies. All the dilutions were made with mobile phase.
Preparation of Sample: Twenty tablets were powdered and average weight equivalent to one tablet was weighed and dissolved by adding mobile phase and sonicated for 30 min. It was filtered to remove the matrix by using Whatmann filter paper of pore size 1 μ and made up the volume to 100 ml with mobile phase (solution A). From solution A, 1 ml was taken and made up the volume to 10 ml with mobile phase (solution B).
- Procedure: Separation of compounds was carried out by using reverse phase columns using Hypersil-BDS C18 (250×4.6 mm. 5 μ) column was selected as the stationary phase, with a flow rate maintained at 1 ml/min with isocratic solvent pumping system. The analysis was done at ambient temperature (~20°). 20 μl of sample was injected and checked at wavelength of 253 nm.
The primary target in developing this method is to achieve simultaneous determination of DEC and LEVC in the tablet formulation under common conditions that will be applicable for routine quality control of the product in laboratories. Various mobile phases such as potassium dihydrogen orthophosphate, Acetonitrile of pH 5.0 with 20:80 ratios were used as mobile phase. At 20:80 ratios, the peaks were eluted at 2.047 and 5.540 min with symmetric and well retained Peaks. For the present study 20:80 ratio was selected.
Effect of flow rate Flow rates of 0.9 and 1.1 ml/min were used and chromatograms were recorded. All these flow rates gave symmetric and well retained peak. For the present study the flow rate 1.0 ml/min was selected. Finally the present mobile phase with flow rate of 1ml/min was used for method development. The ionization of drugs takes place at pH 5. The UV wavelength was optimized at 253 nm for both detection and quantification. At this wavelength both drugs gave significant absorption. No significant peaks were observed from the formulation matrix, indicating no interference from the matrix of the formulation. By this method the peaks were better resolved with retention time’s 2.047±0.05 min for DEC, 5.540±0.02 min for LEVC.
The present method was validated as per ICH guidelines. The peak purity of DEC, LEVC were assessed by comparing the retention times (RT) of standard DEC, LEVC and IS. Good correlation was also found between the retention times of standards and sample of DEC, LEVC.
FIG 1: STANDARD CHROMATOGRAM OF DEC AND LEVC
FIG 2: SAMPLE CHROMATOGRAM OF DEC AND LEVC
Linearity of the responses of the two drugs were verified at six different concentration levels ranging from 20to120 μg/ml for DEC and 0.8 to 4.8 μg/ml for LEVC, respectively. The calibration curve was constructed by plotting response factor (F) against concentration (C) of each drug.
The developed method was applied in the estimation (assay) of DEC and LEVC in tablets. Two batches of the tablets were assayed and results are shown, indicating that the amount of each drug in tablet samples met with requirements (90 to110% of label claim for DEC and 90 to 110% of label claim for LEVC, respectively.
Accuracy: Accuracy of the method was determined in terms of recovery by spiking to the pre-analyzed sample of two different concentrations 100 μg of DEC and 4.0 μg of LEVC standard drugs and the mixtures were reanalyzed by this method for three times.
FIG 3: LINEARITY GRAPHS FOR DEC AND LEVC
Accuracy of DEC:
TABLE 1: ACCURACY RESULTS OF DEC
SamplePerformed | Mean Sample peak area | Mean Standard peak area | Amount taken(µg/ml) | Amount found(µg/ml) | % Recovery |
(80+10) | 1896.833 | 2105.969 | 90 | 89.5645 | 99.51611 |
(100+10) | 2310.812 | 110 | 109.9256 | 99.92323 | |
(120+10) | 2801.135 | 130 | 129.3773 | 99.52103 |
Accuracy of LEVC:
TABLE 2: ACCURACY STUDIES OF LEVC
SamplePerformed | Mean Sample peak area | Mean Standard peak area | Amount taken(µg/ml) | Amount found(µg/ml) | % Recovery |
(3.2+0.4) | 608.118 | 682.7173 | 3.6 | 3.569183 | 99.14398 |
(4.0+0.4) | 754.921 | 4.4 | 4.370849 | 99.33749 | |
(4.8+0.4) | 869.345 | 5.2 | 5.160354 | 99.23758 |
Precision: Precision study was performed to find out system and method precision variations in the estimation of DEC and LEVC of different concentrations, with the proposed method it was found that % RSD is not more than 1% (%RSD of DEC and LEVC are 0.3 and 0.5 respectively in system precision and 0.47 and 0.82 respectively in method precision); as these results are within the range of limits, which indicates that the proposed method has good reproducibility. The results are good for both method precession and system precision.
System Precision of DEC and LEVC:
TABLE 3: SYSTEM PRECISION STUDIES OF DEC AND LEVC
Concentration100% | Injection | Peak Areas ofDEC | Cal. Amount (µg/ml) of DEC | Peak Areas ofLEVC | Cal. Amount (µg/ml) of LEVC |
1 | 2104.820 | 100.26 | 683.129 | 4.02 | |
2 | 2111.902 | 100.58 | 676.891 | 3.98 | |
3 | 2102.853 | 100.16 | 680.579 | 4.01 | |
4 | 2089.405 | 99.85 | 682.247 | 4.02 | |
5 | 2090.124 | 99.90 | 681.987 | 4.01 | |
StatisticalAnalysis | Mean | 2099.821 | 100.15 | 680.967 | 4.008 |
SD | 0.29 | 0.02 | |||
% RSD | 0.29 | 0.5 |
Method Precision of DEC and LEVC:
TABLE 4: METHOD PRECISION RESULTS OF DEC AND LEVC
Concentration100% | Injection | Peak Areas ofDEC | Cal. Amount (µg/ml) ofDEC | Peak Areas of LEVC | Cal. Amount (µg/ml) of LEVC |
1 | 2099.921 | 100.02 | 678.246 | 3.99 | |
2 | 2103.007 | 100.21 | 687.274 | 4.05 | |
3 | 2072.828 | 99.07 | 683.923 | 4.02 | |
4 | 2102.756 | 100.16 | 688.614 | 4.06 | |
5 | 2086.005 | 99.71 | 675.818 | 3.98 | |
StatisticalAnalysis | Mean | 2092.903 | 99.834 | 682.775 | 4.02 |
SD | 0.47 | 0.035 | |||
% RSD | 0.47 | 0.82 |
LOD and LOQ: The LOQ's by this method were found to be as 7.42 and 0.026 ppm for DEC and LEVC respectively. Each value was verified by six individual injections of respective drug. The LOD's were found to be as 2.42 and 0.08 ppm for DEC and LEVC, respectively. These were confirmed by injecting respective standard drug solution at respective concentration for six times.
Ruggedness: Ruggedness was determined on different HPLC systems such as Waters 2487 dual wavelength absorbance detector with a Rheodyne 7725i, 20 μl loop using breeze as data station having 1515 solvent delivery system and a Shimadzu gradient system SPD M-10AVP photo diode array (PDA) detector with Rheodyne 7725i, 20μl loop possessing LC-10 AT VP solvent delivery system using a Class-VP data station with different operators and different stationary phases § Inertsil C18 (250×4.6 mm i.d., 5μ), Hypersil-BDS C18(250×4.6 mm i.d., 5μ) were used and the chromatograms were recorded.
TABLE 5: RUGGEDNESS RESULTS OF DEC AND LEVC
S. NO. | ANALYST-1 | ANALYST-2 | ||||||
Drug | DEC | LEVC | DEC | LEVC | ||||
Peak area | Cal. conc(ug/ml) | Peak area | Cal. Conc. (ug/ml) | Peak area | Cal. Conc. (ug/ml) | Peak area | Cal. Conc ug/ml | |
2103.378 | 100.18 | 685.765 | 4.04 | 2107.750 | 100.39 | 683.679 | 4.03 | |
2087.765 | 99.76 | 689.125 | 4.06 | 2097.557 | 99.91 | 681.146 | 4.02 | |
2104.335 | 100.24 | 677.564 | 3.99 | 2089.672 | 99.88 | 688.256 | 4.05 | |
2099.528 | 100.01 | 680.298 | 4.01 | 2105.632 | 100.29 | 701.572 | 4.13 | |
2097.258 | 99.89 | 686.923 | 4.04 | 2096.598 | 99.83 | 678.832 | 3.99 | |
Mean | 2098.453 | 100.016 | 691.735 | 4.02 | 2099.442 | 100.06 | 691.897 | 4.04 |
SD | 0.2 | 0.027 | 0.26 | 0.04 | ||||
%RSD | 0.2 | 0.67 | 0.26 | 0.9 |
Robustness: Robustness of the method was determined by making slight changes in the chromatographic conditions for change in the ratios of mobile phases, pH and flow rate as mentioned above. . No marked changes were observed. System suitability parameters were checked which include theoretical plate/meter (less than 2500 for both DEC&LEVC), resolution factor (15.873 for LEVC), Tailing factor (1.3 for DEC, 1.5 for LEVC)
TABLE 6: ROBUSTNESS FLOW RATE CHANGE RESULTS OF DEC AND LEVC
S.NO. | Peak area (at flow rate 0.9ml) | Peak area (at flow rate 1.1ml) | ||||||
DEC | LEVC | Conc | DEC | LEVC | Conc | |||
2119.822 | 101.3 | 692.976 | 4.09 | 2100.057 | 100.06 | 712.826 | 4.2 | |
2118.667 | 101.2 | 694.481 | 4.10 | 2104.765 | 100.26 | 714.352 | 4.21 | |
2119.227 | 101.27 | 690.705 | 4.07 | 2103.776 | 100.20 | 709.695 | 4.18 | |
2117.767 | 101.2 | 693.962 | 4.09 | 2105.326 | 100.27 | 711.885 | 4.19 | |
2120.258 | 101.32 | 695.539 | 4.10 | 2102.943 | 100.16 | 713.256 | 4.2 | |
Mean | 2119.148 | 101.258 | 693.533 | 4.09 | 2103.373 | 100.19 | 712.403 | 4.196 |
SD | 0.05 | 0.01 | 0.08 | 0.01 | ||||
%RSD | 0.049 | 0.24 | 0.08 | 0.23 |
TABLE 7: ROBUSTNESS WAVELENGTH CHANGE RESULTS OF DEC AND LEVC
S. NO. | Peak area (at wavelength 252nm) | Peak area (at wavelength 256nm) | ||||||
Drug | DEC | LEVC | DEC | LEVC | Con | |||
2090.931 | 99.53 | 678.438 | 3.9 | 2178.927 | 104 | 551.144 | 3.25 | |
2091.767 | 99.53 | 673.894 | 3.97 | 2177.265 | 103.9 | 549.382 | 3.24 | |
2094.109 | 99.71 | 675.736 | 3.98 | 2177.176 | 103.9 | 554.476 | 3.27 | |
2092.842 | 99.59 | 677.649 | 3.99 | 2180.206 | 104 | 555.023 | 3.28 | |
2097.953 | 99.89 | 680.611 | 4.01 | 2179.953 | 104.1 | 558.194 | 3.3 | |
Mean | 2093.52 | 99.65 | 677.2656 | 3.97 | 2178.705 | 103.9 | 553.644 | 3.27 |
SD | 0.15 | 0.04 | 0.08 | 0.02 | ||||
%RSD | 0.15 | 1.0 | 0.07 | 0.6 |
System Suitability: System suitability parameters can be defined as tests to ensure that the method can generate results of acceptable accuracy and precision. The requirements for system suitability are usually developed after method development and validation has been completed. (Or) The USP (2000) defines parameters that can be used to determine system suitability prior to analysis. The system suitability parameters like Theoretical plates (N), Resolution (R), Tailing factor (T) were calculated and compared with the standard values to ascertain whether the proposed RP-HPLC method for the estimation of DEC and LEVC was validated or not. The results were shown
TABLE 8: SYSTEM SUITABILITY RESULTS OF DEC AND LEVC
Drug | Retention Time (min) | Peak area | USP Plate count | USP Tailing | Resolution |
DIETHYLCARBAMAZINE CITRATE | 2.060 | 2102.082 | 2727 | 1.3 | - |
CETRIZINE DIHYDROCHLORIDE | 5.657 | 701.129 | 7879 | 1.5 | 15.873 |
Assay: Weigh not less than ten capsules. Accurately weigh and transfer powder equivalent to 58.9mg of sample into 50ml volumetric flask, to this 25ml of diluent was added, sonicated and made up to the volume and then filtered. About 10ml of the above solution was made up to 100 mL by using the same diluent.
Calculation:
A Swt Avwt
% Purity = ---------- x ---------- x ----------- x SdP
AS Sdwt Lc
Assay of DEC:
TABLE 9: ASSAY RESULTS OF DEC
Sl. No. | Sample DEC | Peak Area | Cal. Amount (µg/ml) | Statistical Analysis | Standard | Peak Area | Cal. Amount (µg/ml) | Statistical Analysis |
Injection 1 | 2103.007 | 100.16 | SD = 0.17%RSD= 0.17 | Injection 1 | 2102.082 | 100.12 | SD= 0.15%RSD= 0.15 | |
Injection 2 | 2099.756 | 99.92 | Injection 2 | 2103.853 | 100.21 | |||
Average | 2101.381 | 100.04 | Injection 3 | 2098.958 | 99.91 | |||
Average | 2101.631 | 100.08 |
Assay of LEVC:
TABLE 10: ASSAY RESULTS OF LEVC
Sl. No | Sample: LEVC | Peak Area | Cal. Amount (µg/ml) | Statistical Analysis | Standard | Peak Area | Cal. Amount (µg/ml) | Statistical Analysis |
Injection 1 | 697.274 | 4.1 | SD= 0.028%RSD= 0.68 | Injection 1 | 701.129 | 4.13 | SD= 0.03%RSD= 0.73 | |
Injection 2 | 702.614 | 4.14 | Injection 2 | 696.579 | 4.1 | |||
Average | 699.944 | 4.12 | Injection 3 | 689.987 | 4.07 | |||
Average | 696.232 | 4.08 |
SUMMARY AND CONCLUSION: The results showed that the method provided adequate accuracy, precision, sensitivity, reproducibility with better resolution for the analysis of Diethylcarbamazine citrate and Levocetirizine dihydrochloride in formulations either simultaneously or individually. Thus, it can be concluded that the proposed method can be used for the routine analysis of these two drugs in bulk as well as pharmaceutical preparations without any interferences.
ACKNOWLEDGEMENTS: The authors thank the founder of JSS Mahavidyapeetha, Mysore and Dr. B. Suresh, Vice-chancellor, Mysore for providing the facilities to carry out this work.
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How to cite this article:
Movva KV, Jayalakshmi B, Amritharaj RV and Chilamkurthi S: A sensitive RP-HPLC method for simultaneous estimation of Diethylcarbamazine and Levo-Cetirizine in bulk and Pharmaceutical Dosage Form.Int J Pharm Sci Res, 2012; Vol. 3(9): 3347-3353.
Article Information
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3347-3353
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English
IJPSR
Krishna Vamsi Movva*, B. Jayalakshmi, R. Vijay Amritharaj and Sandeep Chilamkurthi
Department of Pharmaceutical Analysis, JKK Munirajah Medical Research Foundation College of Pharmacy, Komarapalayam-638183, Namakkal, Tamil Nadu, India
vamsi729@gmail.com
07 June, 2012
06 July, 2012
27 August, 2012
http://dx.doi.org/10.13040/IJPSR.0975-8232.3(9).3347-53
01 September, 2012