A STUDY OF RELATIONSHIP BETWEEN ANTIBIOTIC RESISTANCE AND MOLECULAR CHARACTERISTICS OF ESCHERICHIA COLI ISOLATES OBTAINED FROM DIFFERENT HUMAN CLINICAL SPECIMENS AGAINST MULTIPLE ANTIBIOTIC RESISTANCE (MAR) INDEX IN BAREILLY (INDIA) REGIONAbstract
To analyse the situation of antibiotic resistance, a total of 77 E. coli isolates from urine, pus, sputum and endo-tracheal aspirate were screened for their antibiograms for antibiotic resistance, multiple antibiotic resistance (MAR) index for evaluating the spread of resistance and plasmid profiles for the presence and characterization of plasmids. Very high resistance level (>90%) was detected against ampicillin, amoxicillin, ceftazidime, norfloxacin, tetracycline while imipenem and amikacin recorded the least resistance levels of 2.3% and 13.9% respectively among the isolates. An increased resistance to amoxicillin, tetracycline, cotrimoxazole and norfloxacin were observed in this geographical area which however displayed a lower resistance in other countries. The MAR index varied considerably, the lowest was 0.18 and the highest was 0.89. Plasmids of 10 size ranges were detected in the isolates. Some isolates possessed single sized plasmid while other possessed multiple plasmids. Isolates with high multi- antibiotic resistance profiles were found to possess multiple plasmids. This study shows that regular antimicrobial sensitivity surveillance is necessary and acquisition of plasmid could greatly contribute in the antibiotic resistance and poses a significant risk of the spread of microbial resistance in this community.
Sandhya Hora* and Ziledar Ali
Central Research Laboratory, Department of Biochemistry, Shri Ram Murti Samarak-Institute of Medical Sciences, Nainital Road, Bareilly-243202, Uttar Pradesh, India
24 May, 2012
07 July, 2012
27 August, 2012
01 September, 2014