A VALIDATED HPLC METHOD FOR ESTIMATION OF LORN AND PCM IN THEIR COMBINED TABLET DOSAGE FORM
AbstractThe present work describes a reverse phase high performance liquid chromatographic method without use of buffer in mobile phase for the simultaneous estimation of Lornoxicam and Paracetamol in combined tablet dosage form. Chromatography was performed on Chromasil C18 (250 × 4.6 mm i.d. and particle size 5 μm) column in isocratic mode with mobile phase containing acetonitrile: 0.2% triethylamine aqueous solution (adjusted to pH 6.54 using 1 % ortho phosphoric acid) in the ratio of 62:38 v/v. The flow rate was 0.8 ml/min and effluents were monitored at 310 nm. The selected chromatographic conditions were found to be useful in separating Lornoxicam (run time 4.00 min) and Paracetamol (run time 3.05 min). Linearity for Lornoxicam and Paracetamol was found to be in the range of 2-14 μg/ml and 250-875 μg/ml with correlation coefficient of 0.9995 and 0.9991 respectively. Percent recovery of the drugs was found to lie between 99.06-99.64 % and 99.58-100.65 % for Lornoxicam and Paracetamol respectively. The proposed method was validated by different parameters. It was found to be accurate, precise, reproducible and specific and hence can be used for simultaneous analysis of these drugs in combined tablet dosage forms.
Article Information
12
1683-1686
510
1008
English
Ijpsr
Sanjay L. Borisagar*, Harsha U. Patel, Ankit N. Patel, and Chhaganbhai N. Patel
Deptartment of Quality Assurance, Shri Sarvajanik Pharmacy College, Near Arvind Baug, B/H- S T Bus stand, Mehsana- 384001, Gujarat, India
26 March, 2011
26 April, 2011
27 June, 2011
http://dx.doi.org/10.13040/IJPSR.0975-8232.2(7).1683-86
01 July, 2011
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